Article Dans Une Revue Methods and Protocols Année : 2026

Decoding Cerebrospinal Fluid: Integrative Metabolomics Across Multiple Platforms

Résumé

Cerebrospinal fluid (CSF) is a key biological matrix that reflects the physiological and pathological states of the central nervous system (CNS). It supports brain function by regulating ionic balance, facilitating molecular transport, and clearing metabolic waste. In this article, we present a standardized protocol for CSF collection along with an integrative multiplatform metabolomic workflow that combines proton nuclear magnetic resonance spectroscopy (1 H-NMRS) and high-performance liquid chromatography coupled to mass spectrometry (HPLC-MS). Integrating these complementary analytical modalities enhances metabolite coverage and improves analytical robustness, enabling a more comprehensive and reliable characterization of the CSF metabolome. This workflow supports the discovery of potential biomarkers and advances our understanding of neurochemical alterations within the CNS.

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inserm-05448355 , version 1 (08-01-2026)

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Antoine Presset, Sylvie Bodard, Antoine Lefèvre, Edward Oujagir, Camille Dupuy, et al.. Decoding Cerebrospinal Fluid: Integrative Metabolomics Across Multiple Platforms. Methods and Protocols, 2026, 9 (1), pp.8. ⟨10.3390/mps9010008⟩. ⟨inserm-05448355⟩
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