Protocols for endothelial cell isolation from mouse tissues: small intestine, colon, heart, and liver
Liliana Sokol
(1, 2, 3)
,
Vincent Geldhof
(1, 2, 3)
,
Melissa García-Caballero
(4, 1, 2, 3)
,
Nadine V Conchinha
(1, 2, 3)
,
Sébastien J Dumas
(1, 2, 3)
,
Elda Meta
(1, 2, 3)
,
Laure-Anne Teuwen
(3, 1, 5, 2, 6)
,
Koen Veys
(1, 2, 3)
,
Rongyuan Chen
(7)
,
Lucas Treps
(8, 1, 2)
,
Mila Borri
(1, 2, 3)
,
Pauline de Zeeuw
(1, 2, 3)
,
Kim D Falkenberg
(1, 2, 3)
,
Charlotte Dubois
(1, 2, 3)
,
Magdalena Parys
(1, 2, 3)
,
Laura P M H de Rooij
(1, 2, 3)
,
Jermaine Goveia
(1, 2, 3)
,
Katerina Rohlenova
(1, 3, 2)
,
Luc Schoonjans
(3, 9, 1, 7)
,
Mieke Dewerchin
(3, 9, 1, 2)
,
Guy Eelen
(3, 1, 2)
,
Xuri Li
(7)
,
Joanna Kalucka
(1, 2, 3, 10)
,
Peter Carmeliet
(1, 2, 3, 10, 7)
1
VIB-KU Leuven | CCB -
VIB-KU Leuven Center for Cancer Biology
2 LKI - Leuven Cancer Institute [Leuven, Belgium]
3 VIB-CCB - Laboratory of Angiogenesis and Vascular Metabolism [Leuven, Belgium]
4 ULiège - Université de Liège = University of Liège = Universiteit van Luik = Universität Lüttich
5 Universiteit Antwerpen = University of Antwerp
6 ZAS Augustinus - GZA Hospitals Sint-Augustinus
7 SYSU - Sun Yat-sen University [Guangzhou]
8 CRCI2NA - Centre de Recherche en Cancérologie et Immunologie Intégrée Nantes-Angers
9 VIB-KU Leuven | CFM - VIB-KU Leuven Center for Microbiology
10 Aarhus University [Aarhus]
2 LKI - Leuven Cancer Institute [Leuven, Belgium]
3 VIB-CCB - Laboratory of Angiogenesis and Vascular Metabolism [Leuven, Belgium]
4 ULiège - Université de Liège = University of Liège = Universiteit van Luik = Universität Lüttich
5 Universiteit Antwerpen = University of Antwerp
6 ZAS Augustinus - GZA Hospitals Sint-Augustinus
7 SYSU - Sun Yat-sen University [Guangzhou]
8 CRCI2NA - Centre de Recherche en Cancérologie et Immunologie Intégrée Nantes-Angers
9 VIB-KU Leuven | CFM - VIB-KU Leuven Center for Microbiology
10 Aarhus University [Aarhus]
Melissa García-Caballero
- Fonction : Auteur
Laure-Anne Teuwen
- Fonction : Auteur
Lucas Treps
- Fonction : Auteur
- PersonId : 1432844
- IdHAL : lucas-treps
- ORCID : 0000-0003-0735-9000
Mieke Dewerchin
- Fonction : Auteur
- PersonId : 1563928
- ORCID : 0000-0002-0382-9346
Peter Carmeliet
- Fonction : Auteur
- PersonId : 1563950
Résumé
Endothelial cells (ECs) from the small intestine, colon, liver, and heart have distinct phenotypes and functional adaptations that are dependent on their physiological environment. Gut ECs adapt to low oxygen, heart ECs to contractile forces, and liver ECs to low flow rates. Isolating high-purity ECs in sufficient quantities is crucial to study their functions. Here, we describe protocols combining magnetic and fluorescent activated cell sorting for rapid and reproducible EC purification from four adult murine tissues. For complete details on the use and execution of these protocols, please refer to Kalucka et al. (2020).
Domaines
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|---|---|
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