5′-AZA induces Ngn3 expression and endocrine differentiation in the PANC-1 human ductal cell line - Inserm - Institut national de la santé et de la recherche médicale Accéder directement au contenu
Article Dans Une Revue Biochemical and Biophysical Research Communications Année : 2010

5′-AZA induces Ngn3 expression and endocrine differentiation in the PANC-1 human ductal cell line

Résumé

Neurogenin 3 is necessary for endocrine cell development in the embryonic pancreas and has been shown to induce transdifferentiation duct cells from adult pancreas toward a neuro-endocrine phenotype. Here we discovered that the demethylating agent 5'-Azadeoxycytidine (AZA) induced Ngn3 expression and endocrine differentiation from the PANC-1 human ductal cell line. The expression of markers specific to mature islet cells, i.e., glucagon and somatostatin, was also observed. In addition, we demonstrated that growth factors (betacellulin and soluble factors released during pancreas embryogenesis) increased the level of maturation. Our studies revealed that the PANC-1 model system may provide a basis for elucidating the ductal/endocrine differentiation.

Dates et versions

inserm-04532368 , version 1 (04-04-2024)

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Citer

Bruno Lefebvre, Sandrine Belaich, Justine Longue, Brigitte Vandewalle, Jose Oberholzer, et al.. 5′-AZA induces Ngn3 expression and endocrine differentiation in the PANC-1 human ductal cell line. Biochemical and Biophysical Research Communications, 2010, 391 (1), pp.305-309. ⟨10.1016/j.bbrc.2009.11.054⟩. ⟨inserm-04532368⟩

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