A photoswitchable inhibitor of TREK channels controls pain in wild-type intact freely moving animals - Inserm - Institut national de la santé et de la recherche médicale Access content directly
Journal Articles Nature Communications Year : 2023

A photoswitchable inhibitor of TREK channels controls pain in wild-type intact freely moving animals

Xavier Gasull
Michael Kienzler
Guillaume Sandoz

Abstract

Abstract By endowing light control of neuronal activity, optogenetics and photopharmacology are powerful methods notably used to probe the transmission of pain signals. However, costs, animal handling and ethical issues have reduced their dissemination and routine use. Here we report LAKI (Light Activated K + channel Inhibitor), a specific photoswitchable inhibitor of the pain-related two-pore-domain potassium TREK and TRESK channels. In the dark or ambient light, LAKI is inactive. However, alternating transdermal illumination at 365 nm and 480 nm reversibly blocks and unblocks TREK/TRESK current in nociceptors, enabling rapid control of pain and nociception in intact and freely moving mice and nematode. These results demonstrate, in vivo, the subcellular localization of TREK/TRESK at the nociceptor free nerve endings in which their acute inhibition is sufficient to induce pain, showing LAKI potential as a valuable tool for TREK/TRESK channel studies. More importantly, LAKI gives the ability to reversibly remote-control pain in a non-invasive and physiological manner in naive animals, which has utility in basic and translational pain research but also in in vivo analgesic drug screening and validation, without the need of genetic manipulations or viral infection.
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Origin : Publication funded by an institution

Dates and versions

inserm-04012034 , version 1 (02-03-2023)

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Arnaud Landra-Willm, Ameya Karapurkar, Alexia Duveau, Anne Amandine Chassot, Lucille Esnault, et al.. A photoswitchable inhibitor of TREK channels controls pain in wild-type intact freely moving animals. Nature Communications, 2023, 14 (1), pp.1160. ⟨10.1038/s41467-023-36806-4⟩. ⟨inserm-04012034⟩
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