Expression and Function of MAO A in Cardiac Cells by Means of Adenovirus-Mediated Gene Transfer - Inserm - Institut national de la santé et de la recherche médicale Access content directly
Book Sections Year : 2023

Expression and Function of MAO A in Cardiac Cells by Means of Adenovirus-Mediated Gene Transfer

Abstract

Gene-transfer methods are useful to study the structural or functional roles of recombinant proteins in vitro. In particular, adenovirus-mediated gene transduction results in strong efficiency and high level of expression in primary cells such as cardiomyocytes, which are difficult to transfect with classical methods. Here, we describe a protocol that enables efficient expression of MAO A in both primary cells and cell lines. Following expression of recombinant MAO A, substrate-induced activation of the enzyme can be assessed by measuring production of reactive oxygen species and downstream signal transduction pathways in cells. This model allows to decipher the biological function of MAO A on metabolism, mitochondrial fitness, cell death/survival, and proliferation.
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inserm-03904055 , version 1 (16-12-2022)

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Yohan Santin, Angelo Parini, Jeanne Mialet-Perez. Expression and Function of MAO A in Cardiac Cells by Means of Adenovirus-Mediated Gene Transfer. Monoamine Oxidase, 2558, Springer US, pp.163-170, 2023, Methods in Molecular Biology, ⟨10.1007/978-1-0716-2643-6_12⟩. ⟨inserm-03904055⟩
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