Angiotensin II Activates Collagen I Gene Through a Mechanism Involving the MAP/ER Kinase Pathway - Inserm - Institut national de la santé et de la recherche médicale Access content directly
Journal Articles Hypertension Year : 2000

Angiotensin II Activates Collagen I Gene Through a Mechanism Involving the MAP/ER Kinase Pathway

Abstract

Abstract —Vascular remodeling and rearrangement of the extracellular matrix formation are among the major adaptive mechanisms to chronic increase in blood pressure. In previous studies we have found that angiotensin II (Ang II) participates in the hypertension-associated aortic and renal vascular fibrosis by stimulating collagen type I formation. The purpose of the present study was to gain insight into the molecular events that lead from the Ang II receptor to collagen I gene activation. To this end, we used a novel strain of transgenic mice harboring the luciferase gene under the control of the collagen I-α 2 chain promoter [procolα 2 (I)]. Ang II produced an early (1 hour) 2- to 3-fold stimulation of procolα 2 (I) activity in freshly isolated aortas and renal cortical slices ( P <0.01) followed by similar increase in procolα 2 (I) mRNA aortic levels. This effect of Ang II was inhibited by AT1-receptor antagonism (candesartan) and blockade of the MAPK/ERK cascade (PD98059); in contrast, inhibition of the P38 kinase pathway (SB202190) and blockade of the release of the transcription factor NFκB (PDTC) did not have any effect in the Ang II–induced activation of the collagen I gene. In addition, Ang II induced a rapid (5 minutes) increase of the MAPK/ERK activity that was accompanied by increased expression (3-fold) of the c- fos proto-oncogene. This increase of c- fos mRNA expression was blocked by PD98059; in addition, curcumin, a blocker of the transcriptional factor AP-1, canceled the effect of Ang II on the collagen I gene. Decorin, a scavenger of the active form of transforming growth factor-β (TGF-β), canceled the Ang II effect on collagen I gene, whereas inhibition of the MAPK/ERK pathway had no effect on the TGF-β–induced activation of procolα 2 (I). These data indicate that the cellular events after AT1 receptor stimulation and leading to activation of collagen I gene expression require activation of both the MAPK/ERK and TGF-β signaling pathways.
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Dates and versions

inserm-03788595 , version 1 (26-09-2022)

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Pierre-Louis Tharaux, Christos Chatziantoniou, Fadi Fakhouri, Jean-Claude Dussaule. Angiotensin II Activates Collagen I Gene Through a Mechanism Involving the MAP/ER Kinase Pathway. Hypertension, 2000, 36 (3), pp.330-336. ⟨10.1161/01.hyp.36.3.330⟩. ⟨inserm-03788595⟩
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