Zwitterionic Polymers towards the Development of Orientation-Sensitive Bioprobes - Inserm - Institut national de la santé et de la recherche médicale Access content directly
Journal Articles Langmuir Year : 2022

Zwitterionic Polymers towards the Development of Orientation-Sensitive Bioprobes


Dynamics with an orientational degree of freedom are fundamental in biological events. Probes with polarized luminescence enable a determination of the orientation. Lanthanide-doped nanocrystals can provide more precise analysis than quantum dots due to the non-photoblinking/bleaching nature and the multiple line-shaped emission. However, the intrinsic polarization property of the original nanocrystals often deteriorates in complex physiological environments, because the colloidal stability easily breaks and the probes aggregate in the media with abundant salts and macromolecules. Engineering the surface chemistry of the probes is thus essential to be compatible with biosystems, which has remained a challenging task that should be exclusively addressed for each specific probe. Here, we demonstrate a facile and efficient surface functionalization of lanthanide-doped nanorods by zwitterionic block copolymers. Due to the steric interaction and the intrinsic zwitterionic nature of the polymers, high colloidal stability of the zwitterionic nanorod suspension is achieved over wide ranges of pH and concentration of salts, even giving rise to the lyotropic liquid crystalline behavior of the nanorods in physiological media. The shear-aligned ability is shown to be unaltered by the coated polymers and thus the strongly polarized emission of Eu 3+ is preserved. Besides, biological experiments reveal good biocompatibility of the zwitterionic nanorods with negligible non-specific binding. This study is a stepping stone for the use of the nanorods as orientation probes in biofluids and validates the strategy of coupling zwitterions to lanthanide-doped nanocrystals for various bio-applications.
Fichier principal
Vignette du fichier
MS.pdf (1.06 Mo) Télécharger le fichier
Origin Files produced by the author(s)

Dates and versions

inserm-03780613 , version 1 (19-09-2022)



Zijun Wang, Fanny Delille, Sophie Bartier, Thomas Pons, Nicolas Lequeux, et al.. Zwitterionic Polymers towards the Development of Orientation-Sensitive Bioprobes. Langmuir, 2022, 38 (34), pp.10512-10519. ⟨10.1021/acs.langmuir.2c01286⟩. ⟨inserm-03780613⟩
61 View
69 Download



Gmail Mastodon Facebook X LinkedIn More