Design and validation of a tunable inertial microfluidic system for the efficient enrichment of circulating tumor cells in blood - Inserm - Institut national de la santé et de la recherche médicale Access content directly
Journal Articles Bioengineering & Translational Medicine Year : 2022

Design and validation of a tunable inertial microfluidic system for the efficient enrichment of circulating tumor cells in blood

Abstract

The analysis of circulating tumor cells (CTCs) in blood is a powerful noninvasive alternative to conventional tumor biopsy. Inertial-based separation is a promising highthroughput, marker-free sorting strategy for the enrichment and isolation of CTCs. Here, we present and validate a double spiral microfluidic device that efficiently isolates CTCs with a fine-tunable cutoff value of 9 μm and a separation range of 2 μm. We designed the device based on computer simulations that introduce a novel, customized inertial force term, and provide practical fabrication guidelines. We validated the device using calibration beads, which allowed us to refine the simulations and redesign the device. Then we validated the redesigned device using blood samples and a murine model of metastatic breast cancer. Finally, as a proof of principle, we tested the device using peripheral blood from a patient with hepatocellular carcinoma, isolating more than 17 CTCs/ml, with purity/removal values of 96.03% and 99.99% of white blood cell and red blood cells, respectively. These results confirmhighly efficient CTC isolation with a stringent cut-off value and better separationresults than the state of the art.
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Origin : Publication funded by an institution

Dates and versions

inserm-03659450 , version 1 (05-05-2022)

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Alejandro Rodríguez‐pena, Estibaliz Armendariz, Alvaro Oyarbide, Xabier Morales, Sergio Ortiz Espinosa, et al.. Design and validation of a tunable inertial microfluidic system for the efficient enrichment of circulating tumor cells in blood. Bioengineering & Translational Medicine, 2022, pp.e10331. ⟨10.1002/btm2.10331⟩. ⟨inserm-03659450⟩
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