Flow cytometric analysis of myelodysplasia: Pre‐analytical and technical issues—Recommendations from the European LeukemiaNet - Inserm - Institut national de la santé et de la recherche médicale Access content directly
Journal Articles Cytometry Part B: Clinical Cytometry Year : 2021

Flow cytometric analysis of myelodysplasia: Pre‐analytical and technical issues—Recommendations from the European LeukemiaNet

Ulrika Johansson
Anna Porwit
Nicolas Chapuis
Michaela Fontenay
Dolores Subirá

Abstract

Background: Flow cytometry (FCM) aids the diagnosis and prognostic stratification of patients with suspected or confirmed myelodysplastic syndrome (MDS). Over the past few years, significant progress has been made in the FCM field concerning technical issues (including software and hardware) and pre-analytical procedures. Methods: Recommendations are made based on the data and expert discussions generated from 13 yearly meetings of the European LeukemiaNet international MDS Flow working group. Results: We report here on the experiences and recommendations concerning (1) the optimal methods of sample processing and handling, (2) antibody panels and fluorochromes, and (3) current hardware technologies. Conclusions: These recommendations will support and facilitate the appropriate application of FCM assays in the diagnostic workup of MDS patients. Further standardization and harmonization will be required to integrate FCM in MDS diagnostic evaluations in daily practice.

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Cancer
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Origin : Publication funded by an institution

Dates and versions

inserm-03540872 , version 1 (24-01-2022)

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Vincent Velden, Frank Preijers, Ulrika Johansson, Theresia Westers, Alan Dunlop, et al.. Flow cytometric analysis of myelodysplasia: Pre‐analytical and technical issues—Recommendations from the European LeukemiaNet. Cytometry Part B: Clinical Cytometry, 2021, Online ahead of print. ⟨10.1002/cyto.b.22046⟩. ⟨inserm-03540872⟩
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