Endogenous PTX3 translocates at the membrane of late apoptotic human neutrophils and is involved in their engulfment by macrophages - Inserm - Institut national de la santé et de la recherche médicale Access content directly
Journal Articles Cell Death and Differentiation Year : 2008

Endogenous PTX3 translocates at the membrane of late apoptotic human neutrophils and is involved in their engulfment by macrophages

Abstract

Neutrophils are short-lived innate immune cells that rapidly die by apoptosis. A rapid and efficient clearance of apoptotic cells is crucial to avoid autoimmunity. This process involves cell alterations, endocytic receptors expressed by phagocytic cells and soluble bridging molecules (opsonins) that facilitate internalization of apoptotic cells by phagocytes. Neutrophils constitutively express the prototypic long pentraxin PTX3 that binds to apoptotic cells and modulates their clearance. We thus evaluated whether endogenous PTX3 may interfere with the capture of apoptotic neutrophils. We observed that PTX3 accumulates in blebs at the surface of late apoptotic neutrophils, resulting from its active translocation from granules to the membrane. A neutralizing anti-PTX3 monoclonal Ab (mAb) inhibits the capture of late apoptotic neutrophils by macrophages. This study shows that intracellular PTX3 translocates at the surface of late apoptotic neutrophils and acts as an 'eat-me' molecule for their recognition and capture by macrophages.

Domains

Immunology
Embargoed file
Embargoed file
Visibility date is undetermined

Dates and versions

inserm-03377801 , version 1 (14-10-2021)

Identifiers

Cite

S Jaillon, P Jeannin, Y Hamon, I Frémaux, A Doni, et al.. Endogenous PTX3 translocates at the membrane of late apoptotic human neutrophils and is involved in their engulfment by macrophages. Cell Death and Differentiation, 2008, 16 (3), pp.465 - 474. ⟨10.1038/cdd.2008.173⟩. ⟨inserm-03377801⟩
12 View
1 Download

Altmetric

Share

Gmail Mastodon Facebook X LinkedIn More