Treatment of Uveitis by In Situ Administration of Ex Vivo–Activated Polyclonal Regulatory T Cells - Inserm - Institut national de la santé et de la recherche médicale Access content directly
Journal Articles Journal of Immunology Year : 2016

Treatment of Uveitis by In Situ Administration of Ex Vivo–Activated Polyclonal Regulatory T Cells

Bruno Lucas

Abstract

CD4(+)CD25(+)Foxp3(+) regulatory T (Treg) cell therapy is a promising approach for the treatment of autoimmune diseases. To be effective, Treg cells should be in an activated state in the target tissue. This can be achieved by systemic administration of Ag-specific Treg cells, which are difficult to produce in conditions that can be translated to the clinic. In this paper, we propose an alternative approach consisting of in situ injection of preactivated polyclonal Treg cells that would exert bystander suppression in the target tissue. We show that polyclonal Treg cells suppressed uveitis in mice as efficiently as Ag-specific Treg cells but only when preactivated and administered in the vitreous. Uveitis control was correlated with an increase of IL-10 and a decrease of reactive oxygen species produced by immune cell infiltrates in the eye. Thus, our results reveal a new mechanism of Treg cell-mediated suppression and a new Treg cell therapy approach.

Domains

Immunology
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Dates and versions

inserm-02368592 , version 1 (18-11-2019)

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Sylvie Grégoire, Céline Terrada, Gaëlle Martin, Gwladys Fourcade, Audrey Baeyens, et al.. Treatment of Uveitis by In Situ Administration of Ex Vivo–Activated Polyclonal Regulatory T Cells. Journal of Immunology, 2016, 196 (5), pp.2109-2118. ⟨10.4049/jimmunol.1501723⟩. ⟨inserm-02368592⟩
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