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Journal Articles Annals of Hematology Year : 2018

International external quality assurance of JAK2 V617F quantification

1 Institute of Biomedicine - Department of Clinical Chemistry and Transfusion Medicine [Gothenburg, Sweden]
2 Department of Hematology [Roskilde, Denmark]
3 Department of Pathology [Barcelona, Spain]
4 Department of Pathology [Odense, Denmark]
5 CHRU Brest - Centre Hospitalier Régional Universitaire de Brest
6 Munich Leukemia Laboratory [Munich, Germany]
7 Institute of Hematology and Blood Transfusion [Prague, Czech Republic]
8 Department of Hematology and Bone Marrow Transplantation [Poznan, Poland]
9 Department of Laboratory Medicine and Pathology [Doha, Qatar]
10 Aarhus University Hospital
11 Rigshospitalet [Copenhagen]
12 LNC - Lipides - Nutrition - Cancer [Dijon - U1231]
13 CHU Dijon - Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand
14 UNIBE - Universität Bern [Bern]
15 Centro di Ricerca e Innovazione per le Malattie Mieloproliferative - CRIMM [ Florence, Italy]
16 Haukeland University Hospital
17 Central Hospital of Southern Pest [Budapest, Hungary]
18 CeMM Research Center for Molecular Medicine [Vienna, Austria]
19 Department of Internal Medicine I [Vienna, Austria]
20 Service de Biologie Cellulaire [Saint-Louis]
21 Clinical Hematology Unit, Hospital Pediátrico [Coimbra, Portugal]
22 Faculty of Pharmacy - Center for Biomolecular Pharmaceutical Analyses [Skopje, Republic of Macedonia]
23 St James’s Hospital [Dublin, Ireland]
24 Dubrava University Hospital - Zagreb School of Medicine [Zagreb, Croatia]
25 Hematology Diagnostics Department - Molecular Diagnostics Laboratory [Krakow, Poland]
26 Hématologie Biologique [CHU de Montpellier]
27 Faculty of Medicine [Bydgoszcz, Poland]
28 CHU Caen
29 Department of Laboratory Medicine [Padova, Italy]
30 Molecular Unit - Department of Hematology & Department of Pathology [Herlev, Denmark]
31 Belfast City Hospital [Belfast, UK]
32 National Research Center for Hematology [Moscow, Russia]
33 CRCINA-ÉQUIPE 16 - Molecular Mechanisms of Chronic Inflammation in Hematological Diseases
34 Laboratoire d'Hematologie [CHU Nantes]
Frank Dicker
  • Function : Author
  • PersonId : 890361
Anni Aggerholm
  • Function : Author
  • PersonId : 887253
Bruno Cassinat
  • Function : Author
  • PersonId : 889796
Dina Naguib
  • Function : Author


External quality assurance (EQA) programs are vital to ensure high quality and standardized results in molecular diagnostics. It is important that EQA for quantitative analysis takes into account the variation in methodology. Results cannot be expected to be more accurate than limits of the technology used, and it is essential to recognize factors causing substantial outlier results. The present study aimed to identify parameters of specific importance for JAK2 V617F quantification by quantitative PCR, using different starting materials, assays, and technical platforms. Sixteen samples were issued to participating laboratories in two EQA rounds. In the first round, 19 laboratories from 11 European countries analyzing JAK2 V617F as part of their routine diagnostics returned results from in-house assays. In the second round, 25 laboratories from 17 countries participated. Despite variations in starting material, assay setup and instrumentation the laboratories were generally well aligned in the EQA program. However, EQA based on a single technology appears to be a valuable tool to achieve standardization of the quantification of JAK2 V617F allelic burden.
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Dates and versions

inserm-01968802 , version 1 (03-01-2019)



Julia Asp, Vibe Skov, Beatriz Bellosillo, Thomas Kristensen, Eric Lippert, et al.. International external quality assurance of JAK2 V617F quantification. Annals of Hematology, 2018, Epub ahead of print. ⟨10.1007/s00277-018-3570-8⟩. ⟨inserm-01968802⟩
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