A filter affinity transfer method for the analysis of rheumatoid factors. - Inserm - Institut national de la santé et de la recherche médicale Access content directly
Journal Articles Journal of Clinical Laboratory Analysis Year : 1991

A filter affinity transfer method for the analysis of rheumatoid factors.

Abstract

Transfer of serum proteins separated by thin layer agarose electrophoresis onto nitrocellulose sheets precoated with purified human polyclonal IgG followed by revelation with enzyme-coupled anti-mu or anti-alpha antisera resulted in the specific detection of rheumatoid factors (RF) belonging to the IgM or IgA classes. Mono- or polyclonality of such RF can be evaluated from the patterns of the blots (sharp bands). In addition, their light chain type can be determined using affinity filters coated with a gamma heavy chain disease protein or with IgG Fc fragments. This simple and rapid procedure allows an easy characterization of monoclonal RF, even if they are present in minute amount amongst polyclonal RF as in certain sera from rheumatoid arthritis patients.
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Dates and versions

inserm-01899804 , version 1 (19-10-2018)

Identifiers

  • HAL Id : inserm-01899804 , version 1
  • PUBMED : 1774602

Cite

Pierre Aucouturier, Jean-Paul Maillochon, Guy Joseph-Théodore, Françoise Duarte, Jean-Louis Preud'Homme. A filter affinity transfer method for the analysis of rheumatoid factors.. Journal of Clinical Laboratory Analysis, 1991, 5 (6), pp.378-81. ⟨inserm-01899804⟩

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