1H-13C NMR-based urine metabolic profiling in autism spectrum disorders. - Inserm - Institut national de la santé et de la recherche médicale Access content directly
Journal Articles Talanta Year : 2013

1H-13C NMR-based urine metabolic profiling in autism spectrum disorders.

Lydie Nadal-Desbarats
Hélène Blasco
Catherine Barthélémy
  • Function : Author
  • PersonId : 885882
  • IdRef : 033754209
Pierre Sarda
  • Function : Author
Patrick Vourc'H
Christian R Andres
Patrick Emond


Autism Spectrum Disorders (ASD) are a group of developmental disorders caused by environmental and genetic factors. Diagnosis is based on behavioral and developmental signs detected before 3 years of age with no reliable biological marker. The purpose of this study was to evaluate the potential use of a 2D NMR-based approach to express the global biochemical signature of autistic individuals compared to normal controls. This technique has greater spectral resolution than to 1D (1)H NMR spectroscopy, which is limited by overlapping signals. The urinary metabolic profiles of 30 autistic and 28 matched healthy children were obtained using a (1)H-(13)C NMR-based approach. The data acquired were processed by multivariate orthogonal partial least-squares discriminant analysis (OPLS-DA). Some discriminating metabolites were identified: β-alanine, glycine, taurine and succinate concentrations were significatively higher, and creatine and 3-methylhistidine concentrations were lower in autistic children than in controls. We also noted differences in several other metabolites that were unidentified but characterized by a cross peak correlation in (1)H-(13)C HSQC. Statistical models of (1)H and (1)H-(13)C analyses were compared and only 2D spectra allowed the characterization of statistically relevant changes [R(2)Y(cum)=0.78 and Q(2)(cum)=0.60] in the low abundance metabolites. This method has the potential to contribute to the diagnosis of neurodevelopment disorders but needs to be validated on larger cohorts and on other developmental disorders to define its specificity.
Fichier principal
Vignette du fichier
TAL-revisedfinal.pdf (389.9 Ko) Télécharger le fichier
Origin Files produced by the author(s)

Dates and versions

inserm-00908985 , version 1 (25-11-2013)



Sylvie Mavel, Lydie Nadal-Desbarats, Hélène Blasco, Frédérique Bonnet-Brilhault, Catherine Barthélémy, et al.. 1H-13C NMR-based urine metabolic profiling in autism spectrum disorders.. Talanta, 2013, 114, pp.95-102. ⟨10.1016/j.talanta.2013.03.064⟩. ⟨inserm-00908985⟩
279 View
427 Download



Gmail Mastodon Facebook X LinkedIn More