HDAC8 mutations in Cornelia de Lange syndrome affect the cohesin acetylation cycle.
Matthew A. Deardorff
(1, 2)
,
Masashige Bando
(3)
,
Ryuichiro Nakato
(3)
,
Erwan Watrin
(4)
,
Takehiko Itoh
(5)
,
Masashi Minamino
(3)
,
Katsuya Saitoh
(3)
,
Makiko Komata
(3)
,
Yuki Katou
(3)
,
Dinah Clark
(1)
,
Kathryn E. Cole
(6)
,
Elfride de Baere
(7)
,
Christophe Decroos
(6)
,
Nataliya Di Donato
(8)
,
Sarah Ernst
(1)
,
Lauren J. Francey
(1)
,
Yolanda Gyftodimou
(9)
,
Kyotaro Hirashima
(10)
,
Melanie Hullings
(1)
,
Yuuichi Ishikawa
(11)
,
Christian Jaulin
(4)
,
Maninder Kaur
(1)
,
Tohru Kiyono
(12)
,
Patrick M. Lombardi
(6)
,
Laura Magnaghi-Jaulin
(4)
,
Geert R. Mortier
(13)
,
Naohito Nozaki
(14)
,
Michael B. Petersen
(15, 16)
,
Hiroyuki Seimiya
(10)
,
Victoria M. Siu
(17)
,
Yutaka Suzuki
(18)
,
Kentaro Takagaki
(19)
,
Jonathan J. Wilde
(1)
,
Patrick J. Willems
(20)
,
Claude Prigent
(4)
,
Gabriele Gillessen-Kaesbach
(21, 22)
,
David W. Christianson
(6)
,
Frank J. Kaiser
(22)
,
Laird G. Jackson
(1, 23)
,
Toru Hirota
(18)
,
Ian D. Krantz
(1, 2)
,
Katsuhiko Shirahige
(3, 24)
1
Division of Human Genetics and Molecular Biology
2 Department of Pediatrics
3 Research Center for Epigenetic Disease
4 IGDR - Institut de Génétique et Développement de Rennes
5 School of Bioscience and Biotechnology
6 Department of Chemistry
7 Center for Medical Genetics [Ghent]
8 Institut für Klinische Genetik
9 Department of Genetics
10 Division of Molecular Biotherapy
11 Department of Pathology
12 Division of Virology
13 Department of Medical Genetics
14 Bio-Frontier Research Center
15 Department of Genetics
16 Department of Clinical Genetics
17 Department of Medical Genetics
18 Graduate School of Frontier Sciences
19 Department of Experimental Pathology
20 GENDIA - GENetic DIAgnostic Network
21 Department of Clinical Genetics
22 Institut für Humangenetik Lübeck
23 Department of Obstetrics and Gynecology
24 CREST - Core Research for Evolutional Science and Technology
2 Department of Pediatrics
3 Research Center for Epigenetic Disease
4 IGDR - Institut de Génétique et Développement de Rennes
5 School of Bioscience and Biotechnology
6 Department of Chemistry
7 Center for Medical Genetics [Ghent]
8 Institut für Klinische Genetik
9 Department of Genetics
10 Division of Molecular Biotherapy
11 Department of Pathology
12 Division of Virology
13 Department of Medical Genetics
14 Bio-Frontier Research Center
15 Department of Genetics
16 Department of Clinical Genetics
17 Department of Medical Genetics
18 Graduate School of Frontier Sciences
19 Department of Experimental Pathology
20 GENDIA - GENetic DIAgnostic Network
21 Department of Clinical Genetics
22 Institut für Humangenetik Lübeck
23 Department of Obstetrics and Gynecology
24 CREST - Core Research for Evolutional Science and Technology
Erwan Watrin
- Fonction : Auteur
- PersonId : 738483
- IdHAL : erwan-watrin
- ORCID : 0000-0002-4582-6493
- IdRef : 075938030
Christophe Decroos
- Fonction : Auteur
- PersonId : 181357
- IdHAL : cdecroos
- ORCID : 0000-0002-2610-3484
- IdRef : 152500677
Claude Prigent
- Fonction : Auteur
- PersonId : 891
- IdHAL : claude-prigent
- ORCID : 0000-0001-8515-8699
- IdRef : 057242747
Katsuhiko Shirahige
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- Fonction : Auteur correspondant
- PersonId : 946516
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Résumé
Cornelia de Lange syndrome (CdLS) is a dominantly inherited congenital malformation disorder, caused by mutations in the cohesin-loading protein NIPBL for nearly 60% of individuals with classical CdLS, and by mutations in the core cohesin components SMC1A (~5%) and SMC3 (<1%) for a smaller fraction of probands. In humans, the multisubunit complex cohesin is made up of SMC1, SMC3, RAD21 and a STAG protein. These form a ring structure that is proposed to encircle sister chromatids to mediate sister chromatid cohesion and also has key roles in gene regulation. SMC3 is acetylated during S-phase to establish cohesiveness of chromatin-loaded cohesin, and in yeast, the class I histone deacetylase Hos1 deacetylates SMC3 during anaphase. Here we identify HDAC8 as the vertebrate SMC3 deacetylase, as well as loss-of-function HDAC8 mutations in six CdLS probands. Loss of HDAC8 activity results in increased SMC3 acetylation and inefficient dissolution of the 'used' cohesin complex released from chromatin in both prophase and anaphase. SMC3 with retained acetylation is loaded onto chromatin, and chromatin immunoprecipitation sequencing analysis demonstrates decreased occupancy of cohesin localization sites that results in a consistent pattern of altered transcription seen in CdLS cell lines with either NIPBL or HDAC8 mutations.