The gut microbiota profile is associated with insulin action in humans. - Inserm - Institut national de la santé et de la recherche médicale Access content directly
Journal Articles Acta Diabetologica Year : 2013

The gut microbiota profile is associated with insulin action in humans.

Abstract

The role of the gut microbiota in the induction of metabolic diseases has now been increasingly recognized worldwide. Indeed, a specific gut microbiota has been shown to characterize lean versus obese phenotypes both in humans and mice. We have also recently demonstrated that a precise gut microbiota is associated with the host's responsiveness to a high-fat diet. Therefore, we hypothesized that insulin resistance in humans could also be linked to a specific gut microbiota. To this aim, microbial DNA and RNA were extracted from the appendix contents of insulin-resistant versus insulin-sensitive obese subjects, matched for body mass index and age, and analyzed by DNA- and RNA-DGGE. Microbial DNA analysis showed that the patients fully segregated according to their degree of insulin action. Conversely, microbial RNA investigation showed that some degree of homology still existed between insulin-sensitive and insulin-resistant patients. Quantitative trait analysis, ordinary least squares regression, principal components regression, partial least squares, canonical correlation analysis, and canonical correspondence analysis also showed a net separation of the two phenotypes analyzed. We conclude that a specific gut microbial profile is associated with insulin action in humans.

Domains

Genetics
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Dates and versions

inserm-00726167 , version 1 (29-08-2012)

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Cite

Matteo Serino, José Manuel Fernández-Real, Eduardo García Fuentes, Maribel Queipo-Ortuño, José María Moreno-Navarrete, et al.. The gut microbiota profile is associated with insulin action in humans.. Acta Diabetologica, 2013, 50 (5), pp.753-61. ⟨10.1007/s00592-012-0410-5⟩. ⟨inserm-00726167⟩
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