Pre-binding of small protein B to a stalled ribosome triggers trans-translation. - Inserm - Institut national de la santé et de la recherche médicale Access content directly
Journal Articles Journal of Biological Chemistry Year : 2004

Pre-binding of small protein B to a stalled ribosome triggers trans-translation.

Abstract

To rescue stalled ribosomes, eubacteria employ a molecule, transfer messenger RNA (tmRNA), which functions both as a tRNA and as an mRNA. With the help of small protein B (SmpB), tmRNA restarts protein synthesis and adds by the trans-translation mechanism a peptide tag to the stalled protein to target it for destruction by cellular proteases. Here, the cellular location and expression of endogenous SmpB were monitored in vivo. We report that SmpB is associated with 70S ribosomes and not in the soluble fraction, independently of the presence of tmRNA. In vitro, SmpB that is pre-bound to a stalled ribosome can trigger initiation of trans-translation. Our results demonstrate the existence of a novel pathway for the entry of tmRNA to the ribosome and for the trans-transfer of a nascent peptide chain from peptidyl-tRNA to charged tmRNA.
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Dates and versions

inserm-00715041 , version 1 (06-07-2012)

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Marc Hallier, Natalia Ivanova, Armelle Rametti, Michael Pavlov, Måns Ehrenberg, et al.. Pre-binding of small protein B to a stalled ribosome triggers trans-translation.. Journal of Biological Chemistry, 2004, 279 (25), pp.25978-85. ⟨10.1074/jbc.M314086200⟩. ⟨inserm-00715041⟩
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