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Journal Articles Methods in Molecular Biology Year : 2009

Intrabody expression in eukaryotic cells.

Abstract

We describe procedures for intracellular expression of scFv in eukaryotic cells. Starting from a scFv gene cloned in a phage-display vector, we describe the cloning step into a mammalian expression vector, the transient transfection of a HeLa cell line, and the monitoring of intrabody expression by immunofluorescence staining and FACS analysis.
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Dates and versions

inserm-00418821 , version 1 (21-09-2009)

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Laurence Guglielmi, Pierre Martineau. Intrabody expression in eukaryotic cells.. Methods in Molecular Biology, 2009, 562, pp.195-203. ⟨10.1007/978-1-60327-302-2_15⟩. ⟨inserm-00418821⟩
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