Profiling of adipokines secreted from human subcutaneous adipose tissue in response to PPAR agonists. - Inserm - Institut national de la santé et de la recherche médicale Access content directly
Journal Articles Biochemical and Biophysical Research Communications Year : 2007

Profiling of adipokines secreted from human subcutaneous adipose tissue in response to PPAR agonists.

Abstract

The role of PPARs in the regulation of human adipose tissue secretome has received little attention despite its potential importance in the therapeutic actions of PPAR agonists. Here, we have investigated the effect of selective PPARgamma, PPARalpha, and PPARbeta/delta agonists on the production of adipokines by human subcutaneous adipose tissue. Antibody arrays were used to measure secreted factors in media from cultured adipose tissue explants. Sixteen proteins were produced in significant amounts. Activation of PPARs regulated the production of five proteins. Treatments with the three PPAR agonists decreased the secretion of leptin and interleukin-6. PPARalpha and beta/delta agonists markedly enhanced hepatocyte growth factor secretion whereas PPARbeta/delta down-regulated angiogenin and up-regulated TIMP-1 release. Hepatocyte growth factor, interleukin-6, and TIMP-1 are chiefly expressed in cells from the stromal vascular fraction whereas angiogenin is expressed in both adipocytes and cells from the stromal vascular fraction. Our data show that PPAR agonists modulate secretion of bioactive molecules from the different cell types composing human adipose tissue.

Dates and versions

inserm-00409656 , version 1 (11-08-2009)

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Eva Klimcáková, Cédric Moro, Anne Mazzucotelli, Karine Lolmède, Nathalie Viguerie, et al.. Profiling of adipokines secreted from human subcutaneous adipose tissue in response to PPAR agonists.. Biochemical and Biophysical Research Communications, 2007, 358 (3), pp.897-902. ⟨10.1016/j.bbrc.2007.05.012⟩. ⟨inserm-00409656⟩
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