Validation of an appropriate reference gene for normalization of reverse transcription-quantitative polymerase chain reaction data from rectal cancer biopsies. - Inserm - Institut national de la santé et de la recherche médicale Access content directly
Journal Articles Analytical Biochemistry Year : 2009

Validation of an appropriate reference gene for normalization of reverse transcription-quantitative polymerase chain reaction data from rectal cancer biopsies.

Abstract

Gene expression quantification using reverse transcription-quantitative polymerase chain reaction (RT-qPCR) requires data normalization using an invariable reference gene. Here we assessed the stability of 15 housekeeping genes in 31 tumor and normal rectal samples to validate a reliable reference gene for rectal cancer studies. Our data show that 18S and 28S RNA are stably expressed in all samples. Moreover, when used for normalization, 18S, but not 28S, greatly reduced unspecific variations of gene expression due to RNA degradation. These results demonstrate that 18S is an appropriate reference gene for normalization of RT-qPCR data from rectal cancer samples.
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Origin : Files produced by the author(s)
Origin : Files produced by the author(s)
Origin : Files produced by the author(s)

Dates and versions

inserm-00396213 , version 1 (19-06-2009)

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Cite

Alexandre Ho-Pun-Cheung, Caroline Bascoul-Mollevi, Eric Assénat, Frédéric Bibeau, Florence Boissière-Michot, et al.. Validation of an appropriate reference gene for normalization of reverse transcription-quantitative polymerase chain reaction data from rectal cancer biopsies.: Normalization of RT-qPCR data in rectal cancer. Analytical Biochemistry, 2009, 388 (2), pp.348-50. ⟨10.1016/j.ab.2009.03.001⟩. ⟨inserm-00396213⟩
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