An automated home-built low-cost fermenter suitable for large-scale bacterial expression of proteins in Escherichia coli. - Inserm - Institut national de la santé et de la recherche médicale Access content directly
Journal Articles Biotechniques Year : 2008

An automated home-built low-cost fermenter suitable for large-scale bacterial expression of proteins in Escherichia coli.

Abstract

We have developed an automated fermentation system for cost-efficient upscaling of protein expression in bacteria. The system, built for use by nonbiotechnologists, can be assembled mostly from standard laboratory equipment and allows a largely unattended growth of bacteria to OD 25 (at 600 nm) in a 12 L vessel. The typical yield of 250-350 g of wet weight cell pellet per run, which is equivalent to the biomass obtained from 250 shake flask cultures containing 400 mL Luria-Broth medium each, facilitates the production of large amounts of purified recombinant protein without the laborious need for optimization of expression and purification conditions.

Dates and versions

inserm-00390951 , version 1 (03-06-2009)

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Uwe Riek, Roland D. Tuerk, Theo Wallimann, Uwe Schlattner, Dietbert Neumann. An automated home-built low-cost fermenter suitable for large-scale bacterial expression of proteins in Escherichia coli.. Biotechniques, 2008, 45 (2), pp.187-9. ⟨10.2144/000112830⟩. ⟨inserm-00390951⟩

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