Cytoplasmic cellular structures control permeability of outer mitochondrial membrane for ADP and oxidative phosphorylation in rat liver cells. - Inserm - Institut national de la santé et de la recherche médicale Access content directly
Journal Articles Biochemical and Biophysical Research Communications Year : 1995

Cytoplasmic cellular structures control permeability of outer mitochondrial membrane for ADP and oxidative phosphorylation in rat liver cells.

Abstract

The kinetics of regulation mitochondrial respiration by external ADP in permeabilized hepatocytes was studied further. In digitonin-permeabilized hepatocytes, the apparent Km for ADP in regulation of respiration was decreased from 275 +/- 35 microM in control to 48 +/- 8 microM by a treatment with trypsin (15 min, 0.125 mg/ml). In liver tissue homogenates, trypsin treatment similarly decreased the Km value for ADP. These results show that ADP diffusion in hepatocytes may be retarded due to some unknown cytoplasmic trypsin-sensitive protein factor(s) which may be lost during isolation of mitochondria. Since we have previously reported a limited permeability of the outer mitochondrial membrane in isolated hepatocytes (Saks et al. 1995, Biochem. Biophys. Res. Commun., 208, 919-926), we conclude that an important site of control of respiration in liver cells in vivo is located at the porin channels of the outer mitochondrial membrane.

Dates and versions

inserm-00390247 , version 1 (01-06-2009)

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Cite

Eric M. Fontaine, Christiane M. Keriel, Sylvie Lantuéjoul, Michel Rigoulet, Xavier M Leverve, et al.. Cytoplasmic cellular structures control permeability of outer mitochondrial membrane for ADP and oxidative phosphorylation in rat liver cells.. Biochemical and Biophysical Research Communications, 1995, 213 (1), pp.138-46. ⟨10.1006/bbrc.1995.2108⟩. ⟨inserm-00390247⟩

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