Subcellular speciation analysis of trace element oxidation states using synchrotron radiation micro-X-ray absorption near-edge structure. - Inserm - Institut national de la santé et de la recherche médicale Access content directly
Journal Articles Analytical Chemistry Year : 2007

Subcellular speciation analysis of trace element oxidation states using synchrotron radiation micro-X-ray absorption near-edge structure.

Abstract

Identification of chemical species at a subcellular level is a key to understand the mechanisms involved in the biology of chemical elements. When performed with a microbeam, X-ray absorption near-edge structure (micro-XANES) enables the direct speciation analysis of oxidation states in subcellular compartments avoiding cell fractionation and other preparation steps that might modify the chemical species. Here we report the principal characteristics in terms of spatial resolution, detection limit, reproducibility, and repeatability of a micro-XANES experimental setup based on Kirkpatrick-Baez X-ray focusing optics that maintains high flux of incoming radiation (>10(11) photons/s) at micrometric spatial resolution (1.5 x 4.0 microm2). Applications and limitations of this setup are illustrated by examples of iron and arsenic absorption spectra obtained from the cytosol, nucleus, and mitochondrial network of cultured cells. A better repeatability and sensitivity with no oxidation state modification and minimal beam damage is achieved when cells are analyzed in a frozen hydrated state, as compared to freeze-dried cells. This original experimental setup can now be applied for the direct speciation analysis of most trace elements at the subcellular level.
Embargoed file
Embargoed file
Visibility date is undetermined
Loading...

Dates and versions

inserm-00383852 , version 1 (24-01-2012)

Identifiers

Cite

Thomas Bacquart, Guillaume Devès, Asunción Carmona, Rémi Tucoulou, Sylvain Bohic, et al.. Subcellular speciation analysis of trace element oxidation states using synchrotron radiation micro-X-ray absorption near-edge structure.. Analytical Chemistry, 2007, 79 (19), pp.7353-9. ⟨10.1021/ac0711135⟩. ⟨inserm-00383852⟩

Collections

INSERM CNRS U836
67 View
0 Download

Altmetric

Share

Gmail Facebook X LinkedIn More