Two RNAi complexes, RITS and RDRC, physically interact and localize to noncoding centromeric RNAs. - Inserm - Institut national de la santé et de la recherche médicale Access content directly
Journal Articles Cell Year : 2004

Two RNAi complexes, RITS and RDRC, physically interact and localize to noncoding centromeric RNAs.

Abstract

RNAi-mediated heterochromatin assembly in fission yeast requires the RNA-induced transcriptional silencing (RITS) complex and a putative RNA-directed RNA polymerase (Rdp1). Here we show that Rdp1 is associated with two conserved proteins, Hrr1, an RNA helicase, and Cid12, a member of the polyA polymerase family, in a complex that has RNA-directed RNA polymerase activity (RDRC, RNA-directed RNA polymerase complex). RDRC physically interacts with RITS in a manner that requires the Dicer ribonuclease (Dcr1) and the Clr4 histone methyltransferase. Moreover, both complexes are localized to the nucleus and associate with noncoding centromeric RNAs in a Dcr1-dependent manner. In cells lacking Rdp1, Hrr1, or Cid12, RITS complexes are devoid of siRNAs and fail to localize to centromeric DNA repeats to initiate heterochromatin assembly. These findings reveal a physical and functional link between Rdp1 and RITS and suggest that noncoding RNAs provide a platform for siRNA-dependent localization of RNAi complexes to specific chromosome regions.

Domains

Genetics

Dates and versions

inserm-00332373 , version 1 (20-10-2008)

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Cite

Mohammad R Motamedi, André Verdel, Serafin U Colmenares, Scott A Gerber, Steven P Gygi, et al.. Two RNAi complexes, RITS and RDRC, physically interact and localize to noncoding centromeric RNAs.. Cell, 2004, 119 (6), pp.789-802. ⟨10.1016/j.cell.2004.11.034⟩. ⟨inserm-00332373⟩

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