Cardiac commitment of primate embryonic stem cells - Inserm - Institut national de la santé et de la recherche médicale
Article Dans Une Revue Nature Protocols Année : 2008

Cardiac commitment of primate embryonic stem cells

Résumé

Primate nonhuman and human embryonic stem (ES) cells provide a powerful model of early cardiogenesis. Furthermore, engineering of cardiac progenitors or cardiomyocytes from ES cells offers a tool for drug screening in toxicology or to search for molecules to improve and scale up the process of cardiac differentiation using high-throughput screening technology, as well as a source of cell therapy of heart failure. Spontaneous differentiation of ES cells into cardiomyocytes is however limited. Herein, we describe a simple protocol to commit both rhesus and human ES cells toward a cardiac lineage and to sort out early cardiac progenitors. Primate ES cells are challenged for 4 d with the cardiogenic morphogen bone morphogenetic protein 2 (BMP2) and sorted out using anti-SSEA-1 antibody-conjugated magnetic beads. Cardiac progenitor cells can be generated and isolated in 4 d using this protocol.
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Dates et versions

inserm-00297337 , version 1 (15-07-2008)

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Citer

Julia Leschik, Sonia Stefanovic, Benjamin Brinon, Michel Pucéat. Cardiac commitment of primate embryonic stem cells. Nature Protocols, 2008, 3 (9), pp.1381-1387. ⟨10.1038/nprot.2008.116⟩. ⟨inserm-00297337⟩
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