V1a vasopressin receptor expression is modulated during myogenic differentiation. - Inserm - Institut national de la santé et de la recherche médicale Access content directly
Journal Articles Differentiation / The Journal of the International Society of Differentiation Year : 2008

V1a vasopressin receptor expression is modulated during myogenic differentiation.

Abstract

Neurohypophyseal peptides potently stimulate myogenic differentiation by acting through different receptors of the same family. Here, we show that L6C5 myogenic cells express, at a high density, a single class of V1a Arg8-vasopressin (AVP) receptor. The expression of the vasopressin receptor of type 1a (V1aR) is significantly higher in proliferating myoblasts than in differentiated myotubes. The differentiation-related decrease of V1aR expression was evident both at the mRNA and at the protein level as shown by the reduction of [(3)H]-AVP binding. However, in L6C5 cells transfected with a synthetic construct containing the luciferase gene driven by the 2 kb upstream region of V1aR, we observed a stimulation of the activity of the promoter when the cells were cultured in differentiative medium. The down-regulation of the V1aR correlated with a decreased half-life of its mRNA (half-life 5.86+/-0.74 hr in 10% fetal bovine serum [FBS] versus 3.53+/-0.72 hr in 1% FBS). Cyclosporine A and dexamethasone, but not 5'-azacytidine, treatments of cells in differentiation medium restored the V1aR level to that measured in proliferating L6C5 cells, thus confirming the role of post-transcriptional mechanisms in the modulation of V1aR expression. Taken together, these data show that mRNA stability plays a role in modulating protein expression during the myogenic differentiation process.
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Dates and versions

inserm-00276755 , version 1 (02-05-2008)

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Cite

Monica Alvisi, Valeria de Arcangelis, Letizia Ciccone, Valeria Palombi, Marta Alessandrini, et al.. V1a vasopressin receptor expression is modulated during myogenic differentiation.. Differentiation / The Journal of the International Society of Differentiation, 2008, 76 (4), pp.371-80. ⟨10.1111/j.1432-0436.2007.00231.x⟩. ⟨inserm-00276755⟩
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