Nanopores: maltoporin channel as a sensor for maltodextrin and lambda-phage. - Inserm - Institut national de la santé et de la recherche médicale Access content directly
Journal Articles Journal of Nanobiotechnology Year : 2005

Nanopores: maltoporin channel as a sensor for maltodextrin and lambda-phage.

Abstract

BACKGROUND: To harvest nutrition from the outside bacteria e.g. E. coli developed in the outer cell wall a number of sophisticated channels called porins. One of them, maltoporin, is a passive specific channel for the maltodextrin uptake. This channel was also named LamB as the bacterial virus phage Lambda mis-uses this channel to recognise the bacteria. The first step is a reversible binding followed after a lag phase by DNA injection. To date little is known about the binding capacity and less on the DNA injection mechanism. To elucidate the mechanism and to show the sensitivity of our method we reconstituted maltoporin in planar lipid membranes. Application of an external transmembrane electric field causes an ion current across the channel. Maltoporin channel diameter is around a few Angstroem. At this size the ion current is extremely sensitive to any modification of the channels surface. Protein conformational changes, substrate binding etc will cause fluctuations reflecting the molecular interactions with the channel wall. The recent improvement in ion current fluctuation analysis allows now studying the interaction of solutes with the channel on a single molecular level. RESULTS: We could demonstrate the asymmetry of the bacterial phage Lambda binding to its natural receptor maltoporin. CONCLUSION: We suggest that this type of measurement can be used as a new type of biosensors.

Domains

Biotechnology
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Dates and versions

inserm-00092492 , version 1 (12-09-2006)

Identifiers

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Emir Berkane, Frank Orlik, Alain Charbit, Christophe Danelon, Didier Fournier, et al.. Nanopores: maltoporin channel as a sensor for maltodextrin and lambda-phage.. Journal of Nanobiotechnology, 2005, 3, pp.3. ⟨10.1186/1477-3155-3-3⟩. ⟨inserm-00092492⟩
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