Article Dans Une Revue Journal of Immunology Année : 2002

Different Qualitative and Quantitative Regulation of Vβ TCR Transcripts During Early Acute Allograft Rejection and Tolerance Induction

Résumé

Recently, using a global method of T cell repertoire analysis, we showed that purified naive T cells confronted in vitro with allogeneic APCs in a direct pathway-restricted MLR up-regulate their Vβ mRNAs without exhibiting skewing of complementarity-determining region 3 (CDR3) length distribution. In this report, using this approach, we show in vivo that Vβ transcript regulation and CDR3 length distribution follow the same pattern during acute rejection of MHC-incompatible heart allografts. In contrast, in tolerance induction by priming of recipients with donor cells, the vigorous Vβ mRNA accumulation with Gaussian CDR3 length distribution is abolished, providing a possible explanation for the down-regulation of activated T cells in tolerant animals. In addition, tolerated grafts harbor T cells with a highly altered repertoire, suggestive of self-restricted presentation with some patterns corresponding to previously identified regulatory cells.

Dates et versions

inserm-05028901 , version 1 (10-04-2025)

Identifiants

Citer

Marina Guillet, Sophie Brouard, Katia Gagne, Fabien Sébille, Maria-Cristina Cuturi, et al.. Different Qualitative and Quantitative Regulation of Vβ TCR Transcripts During Early Acute Allograft Rejection and Tolerance Induction. Journal of Immunology, 2002, 168 (10), pp.5088-5095. ⟨10.4049/jimmunol.168.10.5088⟩. ⟨inserm-05028901⟩
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