Light induces sleep in mice by inhibiting the wake-promoting histaminergic system - Inserm - Institut national de la santé et de la recherche médicale
Preprints, Working Papers, ... (Preprint) Year : 2024

Light induces sleep in mice by inhibiting the wake-promoting histaminergic system

Abstract

Sleep regulation depends on the intricate interplay between homeostatic and circadian rhythms synchronized by the light/dark cycle. Light also directly influences sleep, with the sleep center preoptic area (POA) responsible for mediating light-induced sleep in mice. And this light-responsive POA neurons project to multiple wake-promoting neurons including histaminergic neurons in TMN, whereas there is no functional evidence yet of their involvement in light-induced sleep. Through utilizing hM4Di-HDC-cre mice (HDC+/+, n=8) and histidine decarboxylase (HDC-/-, n=7) knockout mice subjected to an ultradian light/dark (LD1:1h:1h) protocol and hM4Di-HDC-cre mice performed a single light pulse protocol, we revealed light pulses exposed during the biological night induced sleep in HDC+/+ and HDC-/- mice, whereas this sleep-inducing effect was significantly weakened in the latter (82.61 vs 23.40 min, p=0.005). Similarly, this decreased sleep-inducing effect of light was also observed in acute inhibition of histamine model (SA vs DCZ: 15.0 vs 6.05 min, p=0.0016). Furthermore, light pulse elicits a significant increase in ECoG delta activity during SWS in HDC+/+, but this phenomenon was not found in HDC-/- mice. Additionally, the sleep-inducing effect of light is time-dependent, with the strongest effects observed at the beginning and end of the night in HDC +/+ mice, but only at the beginning of the night in HDC-/- mice. Moreover, HDC -/- mice had a delayed, transient response to light stimuli compared to HDC+/+ mice. In mice, light acutely induces sleep during the night via the active sleep-promoting system. Our study further provides evidence that light-induced sleep also benefits from the inhibition of the wake-promoting histaminergic system.
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inserm-04709087 , version 1 (25-09-2024)

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  • HAL Id : inserm-04709087 , version 1

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Yanlong Hou, Ni Tang, Sophie Wu, Yufeng Shao, Damien Gervasoni, et al.. Light induces sleep in mice by inhibiting the wake-promoting histaminergic system. 2024. ⟨inserm-04709087⟩
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