A cytoskeleton-membrane interaction conserved in fast-spiking neurons controls movement, emotion, and memory - Inserm - Institut national de la santé et de la recherche médicale
Journal Articles Molecular Psychiatry Year : 2023

A cytoskeleton-membrane interaction conserved in fast-spiking neurons controls movement, emotion, and memory

Abstract

The pathogenesis of schizophrenia is believed to involve combined dysfunctions of many proteins including microtubule-associated protein 6 (MAP6) and Kv3.1 voltage-gated K+ (Kv) channel, but their relationship and functions in behavioral regulation are often not known. Here we report that MAP6 stabilizes Kv3.1 channels in parvalbumin-positive (PV+ ) fast-spiking GABAergic interneurons, regulating behavior. MAP6-/- and Kv3.1-/- mice display similar hyperactivity and avoidance reduction. Their proteins colocalize in PV+ interneurons and MAP6 deletion markedly reduces Kv3.1 protein level. We further show that two microtubule-binding modules of MAP6 bind the Kv3.1 tetramerization domain with high affinity, maintaining the channel level in both neuronal soma and axons. MAP6 knockdown by AAV-shRNA in the amygdala or the hippocampus reduces avoidance or causes hyperactivity and recognition memory deficit, respectively, through elevating projection neuron activity. Finally, knocking down Kv3.1 or disrupting the MAP6-Kv3.1 binding in these brain regions causes avoidance reduction and hyperactivity, consistent with the effects of MAP6 knockdown. Thus, disrupting this conserved cytoskeleton-membrane interaction in fast-spiking neurons causes different degrees of functional vulnerability in various neural circuits.
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Dates and versions

inserm-04704145 , version 1 (20-09-2024)

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Di Ma, Chao Sun, Rahul Manne, Tianqi Guo, Christophe Bosc, et al.. A cytoskeleton-membrane interaction conserved in fast-spiking neurons controls movement, emotion, and memory. Molecular Psychiatry, 2023, 28 (9), pp.3994-4010. ⟨10.1038/s41380-023-02286-7⟩. ⟨inserm-04704145⟩
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