Article Dans Une Revue Methods in Molecular Biology Année : 2021

New Method for the Expansion of Highly Purified Human Regulatory Granzyme B-Expressing B Cells

Résumé

Granzyme B (GZMB)-expressing B cells inhibit CD4+ T-lymphocyte proliferation in a contact- and GZMB-dependent manner, through degradation of TCR zeta or induction of T-cell apoptosis. This regulatory B-cell population is present in human healthy individuals and represents about 1% of circulating B cells. Their small proportion requires the development of expansion methods to enable their study and envision clinical applications. We describe here how to expand GZMB-expressing B cells to obtain more than 90% of highly purified GZMB+ B cells, and the protocol of B/T cells coculture for the evaluation of the suppressive function of the GZMB+ B-cell population.
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Dates et versions

inserm-04574800 , version 1 (14-05-2024)

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Mélanie Chesneau, Hoa Le Mai, Sophie Brouard. New Method for the Expansion of Highly Purified Human Regulatory Granzyme B-Expressing B Cells. Methods in Molecular Biology, 2021, Methods in Molecular Biology, 2270, pp.203-216. ⟨10.1007/978-1-0716-1237-8_11⟩. ⟨inserm-04574800⟩
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