%0 Journal Article %T Poly(ethylene oxide)/Gelatin-Based Biphasic Photocrosslinkable Hydrogels of Tunable Morphology for Hepatic Progenitor Cell Encapsulation %+ Institut des Molécules et Matériaux du Mans (IMMM) %+ Centre de Recherche en Transplantation et Immunologie - Center for Research in Transplantation and Translational Immunology (U1064 Inserm - CR2TI) %+ Team 2 : Cell and gene engineering in tolerance, fertility and regenerative medicine (U1064 Inserm - CR2TI) %+ Institut de Recherche en Santé de l'Université de Nantes (IRS-UN) %A Meng, Yuwen %A Gantier, Malika %A Nguyen, Tuan Huy %A Nicolai, Taco %A Nicol, Erwan %< avec comité de lecture %@ 1525-7797 %J Biomacromolecules %I American Chemical Society %V 24 %N 2 %P 789-796 %8 2023-02-13 %D 2023 %R 10.1021/acs.biomac.2c01250 %M 36655630 %Z Life Sciences [q-bio]Journal articles %X Macroporous hydrogels have great potential for biomedical applications. Liquid or gel-like pores were created in a photopolymerizable hydrogel by forming water-in-water emulsions upon mixing aqueous solutions of gelatin and a poly(ethylene oxide) (PEO)-based triblock copolymer. The copolymer constituted the continuous matrix, which dominated the mechanical properties of the hydrogel once photopolymerized. The gelatin constituted the dispersed phase, which created macropores in the hydrogel. The microstructures of the porous hydrogel were determined by the volume fraction of the gelatin phase. When volume fractions were close to 50 v%, free-standing hydrogels with interpenetrated morphology can be obtained thanks to the addition of a small amount of xanthan. The hydrogels displayed Young's moduli ranging from 5 to 30 kPa. They have been found to be non-swellable and non-degradable in physiological conditions. Preliminary viability tests with hepatic progenitor cells embedded in monophasic PEO-based hydrogels showed rapid mortality of the cells, whereas encouraging viability was observed in PEO-based triblock copolymer/gelatin macroporous hydrogels. The latter has the potential to be used in cell therapy. %G English %L inserm-04077504 %U https://inserm.hal.science/inserm-04077504 %~ INSERM %~ CNRS %~ UNIV-LEMANS %~ INC-CNRS %~ CRTI %~ IMMM-LEMANS %~ NANTES-UNIVERSITE %~ NANTES-UNIV %~ CR2TI %~ UFR-MEDECINE-NANTES