%0 Journal Article %T Increase in PGE2 biosynthesis induces a Bax dependent apoptosis correlated to patients’ survival in glioblastoma multiforme %+ Recherches en cancérologie %+ Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE) %+ Service d'Anatomie Pathologique [CHU Nantes] %+ Service de Neurochirurgie [Nantes] %A Lalier, Lisenn %A Cartron, P-F %A Pedelaborde, F %A Olivier, C %A Loussouarn, D %A Martin, S, A %A Meflah, K %A Menanteau, J %A Vallette, F, M %< avec comité de lecture %@ 0950-9232 %J Oncogene %I Nature Publishing Group %V 26 %P 4999 - 5009 %8 2007-03-19 %D 2007 %R 10.1038/sj.onc.1210303 %M 17369862 %K gliomas %K cyclooxygenase %K prostaglandin synthase %K apoptosis %K Bax %K PGE 2 %Z Life Sciences [q-bio]/CancerJournal articles %X Prostaglandin E 2 plays multiple roles both in the physiology and the physiopathology of human brain, which are not completely understood. We have identified in a subset of human glioblastoma multiforme (GBM) tumors, the most common form of adult brain cancer, an increased expression of mPGES-1, the enzyme which catalyses the isomerization of PGH 2 into PGE 2 downstream of cyclooxygenase 2 (COX-2). The sensitivity of primary cultures of GBM to apoptosis was augmented by the overexpression of mPGES-1, whereas the knockdown of its expression by shRNA decreased the apoptotic threshold in vitro and stimulated tumor growth in vivo. Adding extracellular PGE 2 in the culture medium failed to reproduce mPGES-1 effect on the cell viability in vitro. However, the intracellular injection of PGE 2 induced a dose-dependent apoptosis in GBM cultures, which was dependent on the presence of Bax, a pro-apoptotic protein. We show that PGE 2 physically associates with Bax, triggering its apoptotic-like change in conformation and its subsequent association with mitochondria. Our results raise questions about the role of PGE 2 in the control of apoptosis and in its potential impact in central nervous system pathologies. %G English %2 https://inserm.hal.science/inserm-03369894/document %2 https://inserm.hal.science/inserm-03369894/file/Lalier%20Oncogene07%20%28PGE2%29.pdf %L inserm-03369894 %U https://inserm.hal.science/inserm-03369894 %~ INSERM %~ UNIV-NANTES %~ NANTES-UNIVERSITE %~ UNIV-NANTES-AV2022 %~ UFR-MEDECINE-NANTES