%0 Journal Article %T Cooperation of polarized cell intercalations drives convergence and extension of presomitic mesoderm during zebrafish gastrulation %+ Department of Biological Sciences [Nashville] %+ Biomathematics Study Group [Nashville, TN, USA] (Department of Mathematics) %+ Physico-Chimie-Curie (PCC) %A Yin, Chunyue %A Kiskowski, Maria %A Pouille, Philippe-Alexandre %A Farge, Emmanuel %A Solnica-Krezel, Lilianna %Z This work in the L. Solnica-Krezel laboratory is supported by National Institutes of Health grant GM55101 and Human Frontiers in Science Program (HSFP) RGP14/2006. Work in the E. Farge laboratory is supported by Microsoft Research through the European PhD Scholarship Program, the INSERM and CNRS (ITS2005), and the HSFP. %< avec comité de lecture %@ 0021-9525 %J Journal of Cell Biology %I Rockefeller University Press %V 180 %N 1 %P 221-232 %8 2008-01-14 %D 2008 %R 10.1083/jcb.200704150 %M 18195109 %K PIV %K planar cell polarity %K PCP %K mediolateral %K ML %K membrane %K PSM %K presomitic mesoderm %K prickle %K Pk %K particle image veloci- metry %K extension %K Dsh %K dishevelled %K hpf %K hours post fertilization %K kny %K knypek %K mem %K Stbm %K Strabismus %K tri %K trilobite %K Vangl2 %K Van Gogh-like 2 %K WT %K wild type %Z Life Sciences [q-bio] %Z Life Sciences [q-bio]/Development Biology %Z Life Sciences [q-bio]/Development Biology/Embryology and Organogenesis %Z Life Sciences [q-bio]/Development Biology/MorphogenesisJournal articles %X During vertebrate gastrulation, convergence and extension (C&E) movements narrow and lengthen the embryonic tissues, respectively. In zebrafish, regional differences of C&E movements have been observed; however, the underlying cell behaviors are poorly understood. Using time-lapse analyses and computational modeling, we demonstrate that C&E of the medial presomitic mesoderm is achieved by cooperation of planar and radial cell intercalations. Radial intercalations preferentially separate anterior and posterior neighbors to promote extension. In knypek;trilobite noncanonical Wnt mutants, the frequencies of cell intercalations are altered and the anteroposterior bias of radial intercalations is lost. This provides evidence for noncanonical Wnt signaling polarizing cell movements between different mesodermal cell layers. We further show using fluorescent fusion proteins that during dorsal mesoderm C&E, the noncanonical Wnt component Prickle localizes at the anterior cell edge, whereas Dishevelled is enriched posteriorly. Asymmetrical localization of Prickle and Dishevelled to the opposite cell edges in zebrafish gastrula parallels their distribution in fly, and suggests that noncanonical Wnt signaling defines distinct anterior and posterior cell properties to bias cell intercalations. %G English %2 https://inserm.hal.science/inserm-02440915/document %2 https://inserm.hal.science/inserm-02440915/file/Yin-Solnica%20jcb_200704150.pdf %L inserm-02440915 %U https://inserm.hal.science/inserm-02440915 %~ INSERM %~ UPMC %~ CNRS %~ FNCLCC %~ CURIE %~ PCC_UMR168 %~ INC-CNRS %~ PSL %~ UPMC_POLE_2 %~ SORBONNE-UNIVERSITE %~ SU-SCIENCES %~ INSTITUT-CURIE-PSL %~ SU-TI %~ ALLIANCE-SU