TCTP and CSN4 control cell cycle progression and development by regulating CULLIN1 neddylation in plants and animals - Inserm - Institut national de la santé et de la recherche médicale Access content directly
Journal Articles PLoS Genetics Year : 2019

TCTP and CSN4 control cell cycle progression and development by regulating CULLIN1 neddylation in plants and animals

Abstract

Translationally Controlled Tumor Protein (TCTP) controls growth by regulating the G1/S transition during cell cycle progression. Our genetic interaction studies show that TCTP fulfills this role by interacting with CSN4, a subunit of the COP9 Signalosome complex, known to influence CULLIN-RING ubiquitin ligases activity by controlling CULLIN (CUL) neddylation status. In agreement with these data, downregulation of CSN4 in Arabidopsis and in tobacco cells leads to delayed G1/S transition comparable to that observed when TCTP is downregulated. Loss-of-function of AtTCTP leads to increased fraction of deneddylated CUL1, suggesting that AtTCTP interferes negatively with COP9 function. Similar defects in cell proliferation and CUL1 neddylation status were observed in Drosophila knockdown for dCSN4 or dTCTP, respectively, demonstrating a conserved mechanism between plants and animals. Together, our data show that CSN4 is the missing factor linking TCTP to the control of cell cycle progression and cell proliferation during organ development and open perspectives towards understanding TCTP's role in organ development and disorders associated with TCTP miss-expression.
Fichier principal
Vignette du fichier
journal.pgen.1007899.pdf (3.5 Mo) Télécharger le fichier
Origin : Publication funded by an institution
Loading...

Dates and versions

inserm-02155448 , version 1 (13-06-2019)

Licence

Attribution

Identifiers

Cite

Léo Betsch, Véronique Boltz, Florian Brioudes, Garance Pontier, Victor Girard, et al.. TCTP and CSN4 control cell cycle progression and development by regulating CULLIN1 neddylation in plants and animals. PLoS Genetics, 2019, 15 (1), pp.e1007899. ⟨10.1371/journal.pgen.1007899⟩. ⟨inserm-02155448⟩
100 View
97 Download

Altmetric

Share

Gmail Facebook X LinkedIn More