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Book Sections Year : 2016

Genome Editing in Rats Using TALE Nucleases

Anne de Cian

Abstract

The rat is an important animal model to understand gene function and model human diseases. Since recent years, the development of gene-specific nucleases has become important for generating new rat models of human diseases, to analyze the role of genes and to generate human antibodies. Transcription activator-like (TALE) nucleases efficiently create gene-specific knockout rats and lead to the possibility of gene targeting by homology-directed recombination (HDR) and generating knock-in rats. We describe a detailed protocol for generating knockout and knock-in rats via microinjection of TALE nucleases into fertilized eggs. This technology is an efficient, cost- and time-effective method for creating new rat models.
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Dates and versions

inserm-02150568 , version 1 (07-06-2019)

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Laurent Tesson, Severine Remy, Séverine Ménoret, Claire Usal, Reynald Thinard, et al.. Genome Editing in Rats Using TALE Nucleases. TALENs. Methods in Molecular Biology, pp.245-259, 2016, ⟨10.1007/978-1-4939-2932-0_18⟩. ⟨inserm-02150568⟩
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