%0 Journal Article %T Cluster-determinant 36 (CD36) impacts on vitamin E postprandial response %+ Nutrition, obésité et risque thrombotique (NORT) %+ Physiologie de la Nutrition et Toxicologie (NUTox) (U866, Lipides et nutrition, équipe 7) (NUTox) %A Goncalves, Aurélie %A Roi, Stéphanie %A Nowicki, Marion %A Niot, Isabelle %A Reboul, Emmanuelle %< avec comité de lecture %@ 1613-4125 %J Molecular Nutrition and Food Research %I Wiley-VCH Verlag %V 58 %N 12 %P 2297 - 2306 %8 2014 %D 2014 %R 10.1002/mnfr.201400339 %M 25174330 %K alpha-Tocopherol %K Bioavailability %K CD36 %K Transgenic mice %K gamma-Tocopherol %K Intestinal absorption %Z Life Sciences [q-bio]/Food and NutritionJournal articles %X Scope: A single nucleotide polymorphism in the cluster determinant 36 (CD36) gene has recently been associated with plasma alpha-tocopherol concentration, suggesting a possible role of this protein in vitamin E intestinal absorption or tissue uptake. Methods and results: To investigate the involvement of CD36 in vitamin E transport, we first evaluated the effect of CD36 on alpha- and gamma-tocopherol transmembrane uptake and efflux using transfected HEK cells. gamma-Tocopherol postprandial response was then assessed in CD36-deficient mice compared with wild-type mice, after the mice had been fully characterized for their alpha -tocopherol, vitamin A and lipid plasma, and tissue contents. Both alpha- and gamma-tocopherol uptake was significantly increased in cells overexpressing CD36 compared with control cells. Compared with wild-typemice, CD36-deficient mice displayed a significantly decreased cholesterol hepatic concentration, and males exhibited significantly higher triacylglycerol contents in liver, brain, heart, and muscle. Although tissue alpha -tocopherol concentration after adjustment for lipid content was not modified, gamma-tocopherol postprandial response was significantly increased in CD36-deficient mice compared with controls, likely reflecting the postprandial hypertriglyceridemia observed in these mice. Conclusion: Our findings show for the first time that CD36 participates-directly or indirectly-in vitamin E uptake, and that CD36 effect on postprandial lipid metabolism in turn modifies vitamin E postprandial response. %G English %L inserm-01478595 %U https://inserm.hal.science/inserm-01478595 %~ INSERM %~ UNIV-BOURGOGNE %~ UNIV-AMU %~ INRA %~ LNC-UMR866 %~ AGREENIUM %~ AGROSUP-DIJON %~ ENSBANA %~ UMR866-EQUIPE7 %~ INRAE %~ TEST3-HALCNRS %~ INSTITUT-AGRO-DIJON %~ INSTITUT-AGRO-DIJON-LNC %~ INSTITUT-AGRO-LNC %~ INSTITUT-AGRO %~ PUBLIS-EC-IA