%0 Journal Article %T β-Lactoglobulin as a Vector for β-Carotene Food Fortification %+ Nutrition, obésité et risque thrombotique (NORT) %+ Unité de recherche sur les Biopolymères, Interactions Assemblages (BIA) %A Mensi, Azza %A Borel, Patrick %A Goncalves, Aurélie %A Nowicki, Marion %A Gleize, Béatrice %A Roi, Stéphanie %A Chobert, Jean-Marc %A Haertlé, Thomas %A Reboul, Emmanuelle %Z Ecole Doctorale VENAM (University of Nantes, France) %< avec comité de lecture %@ 0021-8561 %J Journal of Agricultural and Food Chemistry %I American Chemical Society %V 62 %N 25 %P 5916 - 5924 %8 2014-06-25 %D 2014 %R 10.1021/jf501683s %M 24857546 %K retinol %K carotenoid %K emulsion %K vitamin A %K micelles %Z Life Sciences [q-bio]/Food and NutritionJournal articles %X Food fortification is a strategy to overcome vitamin A deficiency in developing countries. Our aim was to investigate the involvement of the bovine milk protein beta-lactoglobulin (beta-Lg), a potential retinoid carrier, in vitamin A absorption. In vivo experiments were conducted by force-feeding mice with retinol or beta-carotene associated with either beta-Lg or oil-in-water emulsion, with subsequent determination of both vitamin A intestinal mucosa and plasma contents. Caco-2 cells were then used to investigate the mechanisms of vitamin A uptake when delivered by either beta-Lg or mixed micelles. We showed that beta-Lg was as efficient as emulsion to promote beta-carotene, but not retinol, absorption in mice. Similar results were obtained in vitro. Interestingly, an inhibitor of the Scavenger Receptor Class B Type I significantly decreased the uptake of micellar beta-carotene but not that of beta-carotene bound to beta-Lg. Overall, we showed that beta-Lg would be a good vector for beta-carotene food fortification. %G English %L inserm-01478504 %U https://inserm.hal.science/inserm-01478504 %~ INSERM %~ UNIV-AMU %~ INRA %~ AGREENIUM %~ INRAE %~ TEST3-HALCNRS