%0 Journal Article %T Toxoplasma gondii: Flat-mounting of retina as a new tool for the observation of ocular infection in mice %+ Immunité et Infection %+ Centre de Recherche des Cordeliers (CRC (UMR_S 872)) %+ Service d'ophtalmologie [Hôtel-Dieu - Paris] %+ CHU Pitié-Salpêtrière [AP-HP] %+ Dynamique des interactions membranaires normales et pathologiques (DIMNP) %A Escoffier, Patricia %A Jeanny, Jean-Claude %A Marinach-Patrice, Carine %A Jonet, Laurent %A Raoul, William %A Behar-Cohen, Francine %A Paris, Luc %A Danis, Martin %A Dubremetz, Jean François %A Mazier, Dominique %Z We thank the doctorate school ‘‘Public Health: Epidemiologyand Biomedical Information Science” (University of Pierre et MarieCurie, Paris, France) for financial support %< avec comité de lecture %@ 0014-4894 %J Experimental Parasitology %I Elsevier %P 259-262 %8 2010-04-19 %D 2010 %R 10.1016/j.exppara.2010.04.006 %M 20412796 %K Macroscopic visualization %K Ocular infection %K Toxoplasma gondii %K Flat-mount of retina %Z Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC] %Z Life Sciences [q-bio]/Human health and pathology/Infectious diseasesJournal articles %X Ocular toxoplasmosis is the principal cause of posterior uveitis and a leading cause of blindness. Animal models are required to improve our understanding of the pathogenesis of this disease. The method cur-rently used for the detection of retinal cysts in animals involves the observation, under a microscope, of all the sections from infected eyes. However, this method is time-consuming and lacks sensitivity. We have developed a rapid, sensitive method for observing retinal cysts in mice infected with Toxoplasma gondii. This method involves combining the flat-mounting of retina – a compromise between macro-scopic observation and global analysis of this tissue – and the use of an avirulent recombinant strain of T. gondii expressing the Escherichia coli b-galactosidase gene, visually detectable at the submacroscopic level. Single cyst unilateral infection was found in six out of 17 mice killed within 28 days of infection, whereas a bilateral infection was found in only one mouse. There was no correlation between brain cysts number and ocular infection. %G English %2 https://inserm.hal.science/inserm-01094666/document %2 https://inserm.hal.science/inserm-01094666/file/2010%20Exp%20Parasitology.pdf %L inserm-01094666 %U https://inserm.hal.science/inserm-01094666 %~ INSERM %~ UNIV-PARIS5 %~ UPMC %~ CNRS %~ UNIV-MONTP1 %~ UNIV-MONTP2 %~ APHP %~ DIMNP %~ CORDELIERS %~ UPMC_POLE_4 %~ BS %~ UNIV-MONTPELLIER %~ SORBONNE-UNIVERSITE %~ SU-INF-2018 %~ SU-MEDECINE %~ SU-SCIENCES %~ SU-MED %~ LPHI %~ UNIV-PARIS %~ SU-TI %~ ALLIANCE-SU %~ UM1-UM2 %~ UM-2015-2021