%0 Journal Article %T Changes in brain tissue oxygenation after treatment of diffuse traumatic brain injury by erythropoietin. %+ INSERM U836, équipe 5, Neuroimagerie fonctionnelle et perfusion cérébrale %+ INSERM U836, équipe 5, Neuroimagerie fonctionnelle et perfusion cérébrale %+ Grenoble Institut des Neurosciences (GIN) %+ Pôle Anesthésie Réanimation %+ Neuro-imagerie fonctionnelle et métabolique (ANTE-INSERM U836, équipe 5) %A Bouzat, Pierre %A Millet, Anne %A Boue, Yvonnick %A Pernet-Gallay, Karin %A Trouve-Buisson, Thibaut %A Gaide-Chevronnay, Lucie %A Barbier, Emmanuel, L. %A Payen, Jean-Francois %Z INSERM; CNRS; Fondation "Gueules cassées"; Association pour la Recherche en Néonatologie; Ministère de l'Education, de la Recherche et de la Technologie; %< avec comité de lecture %@ 0090-3493 %J Critical Care Medicine %I Lippincott, Williams & Wilkins %V 41 %N 5 %P 1316-24 %8 2013-05 %D 2013 %R 10.1097/CCM.0b013e31827ca64e %M 23591210 %Z Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC] %Z Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/NeurobiologyJournal articles %X OBJECTIVES: To investigate the effects of recombinant human erythropoietin on brain oxygenation in a model of diffuse traumatic brain injury. DESIGN: Adult male Wistar rats. SETTING: Neurosciences and physiology laboratories. INTERVENTIONS: Thirty minutes after diffuse traumatic brain injury (impact-acceleration model), rats were intravenously administered with either a saline solution or a recombinant human erythropoietin (5000 IU/kg). A third group received no traumatic brain injury insult (sham-operated). MEASUREMENTS AND MAIN RESULTS: Three series of experiments were conducted 2 hours after traumatic brain injury to investigate: 1) the effect of recombinant human erythropoietin on brain edema using diffusion-weighted magnetic resonance imaging and measurements of apparent diffusion coefficient (n = 11 rats per group); local brain oxygen saturation, mean transit time, and blood volume fraction were subsequently measured using a multiparametric magnetic resonance-based approach to estimate brain oxygenation and brain perfusion in the neocortex and caudoputamen; 2) the effect of recombinant human erythropoietin on brain tissue PO₂ in similar experiments (n = 5 rats per group); and 3) the cortical ultrastructural changes after treatment (n = 1 rat per group). Compared with the sham-operated group, traumatic brain injury saline rats showed a significant decrease in local brain oxygen saturation and in brain tissue PO₂ alongside brain edema formation and microvascular lumen collapse at H2. Treatment with recombinant human erythropoietin reversed all of these traumatic brain injury-induced changes. Brain perfusion (mean transit time and blood volume fraction) was comparable between the three groups of animals. CONCLUSION: Our findings indicate that brain hypoxia can be related to microcirculatory derangements and cell edema without evidence of brain ischemia. These changes were reversed with post-traumatic administration of recombinant human erythropoietin, thus offering new perspectives in the use of this drug in brain injury. %G English %2 https://inserm.hal.science/inserm-00861125/document %2 https://inserm.hal.science/inserm-00861125/file/Bouzat_2013_ChangesIn_AE.pdf %L inserm-00861125 %U https://inserm.hal.science/inserm-00861125 %~ INSERM %~ UGA %~ UNIV-GRENOBLE1 %~ U836