%0 Journal Article %T BCL2 expression in CD105 positive neoangiogenic cells and tumor progression in angioimmunoblastic T-cell lymphoma. %+ Gvh et Gvl : Physiopathologie Chez l'Homme et Chez l'Animal, Incidence et Role Therapeutique %+ Laboratory of Pathogenesis of Leukemia %+ Service d'anatomo-pathologie [Paris] %+ Service d'hématologie-oncologie adultes %A Ratajczak, Philippe %A Leboeuf, Christophe %A Wang, Li %A Brière, Josette %A Loisel-Ferreira, Irmine %A Thiéblemont, Catherine %A Zhao, Wei-Li %A Janin, Anne %< avec comité de lecture %@ 0893-3952 %J Modern Pathology %I Nature Publishing Group: Open Access Hybrid Model Option B %V 25 %N 6 %P 805-14 %8 2012-06 %D 2012 %R 10.1038/modpathol.2012.1 %M 22322190 %K Angioimmunolbastic T-Cell Lyphoma %K BCL2 %K CD105 %K endothelial cell %K neoangiogenesis %K VEGF %Z Life Sciences [q-bio]/CancerJournal articles %X The angiogenic microenvironment has been known to be a component of angioimmunoblastic T-cell lymphoma since its initial characterization. We have shown that angioimmunoblastic T-cell lymphoma endothelial cells produce vascular endothelial growth factor-A (VEGFA), and participate in lymphoma progression. In squamous cell carcinoma, endothelial BCL2 expression induces a crosstalk with tumor cells through VEGFA, a major mediator of tumoral angiogenesis. In the present study, we analyzed BCL2 and VEGFA in 30 angioimmunoblastic T-cell lymphomas, using triple immunofluorescence to identify protein coexpression in well-characterized lymphoma cells and microenvironment neoangiogenic endothelial cells. Using quantitative real-time PCR, we assessed mRNA expression levels in laser-microdissected endothelial and lymphoma cells. In lymphoma cells, as in endothelial cells, BCL2 and VEGFA proteins were coexpressed. BCL2 was expressed only in neoangiogenic CD34(+)CD105(+) endothelial cells. In laser-microdissected cells, mRNA studies showed a significant relationship between BCL2 and VEGFA levels in CD34(+) endothelial cells, but not in CD3(+)CD10(+)lymphoma cells, or in CD34(+) endothelial cells from lymph node hyperplasia. Further study showed that, in AITL, BCL2 mRNA levels in CD34(+)CD105(+) neoangiogenic endothelial cells also correlated with microvessel density, International Prognostic Index, Ann Arbor stage, bone marrow involvement and elevated LDH. BCL2 expression by CD105(+) neoangiogenic endothelial cells is related to tumor progression in angioimmunoblastic T-cell lymphoma. %G English %2 https://inserm.hal.science/inserm-00682931/document %2 https://inserm.hal.science/inserm-00682931/file/Ratajczak-submitted_revised_-Modern_Pathology.pdf %2 https://inserm.hal.science/inserm-00682931/file/inserm-00682931_edited.pdf %L inserm-00682931 %U https://inserm.hal.science/inserm-00682931 %~ INSERM %~ UNIV-PARIS7 %~ APHP %~ UNIV-PARIS