%0 Journal Article %T Differential activity of candidate microbicides against early steps of HIV-1 infection upon complement virus opsonization. %+ Laboratoire de Virologie %+ Développement normal et pathologique des lymphocytes et signalisation %+ Rega Institute for Medical Research [Leuven, België] %+ University of Nevada [Reno] %+ Virology Unit %A Jenabian, Mohammad-Ali %A Saïdi, Héla %A Charpentier, Charlotte %A Bouhlal, Hicham %A Schols, Dominique %A Balzarini, Jan %A Bell, Thomas %A Vanham, Guido %A Bélec, Laurent %Z The study was supported by grants from the European Community (VIth Framework, "EMPRO" project; contract no. 503558), the Agence Nationale de Recherches sur le SIDA et les hépatites virales ("Multi-Micro" project) and the Centers of Excellence of the K.U. Leuven (contract no. 05/15). Hela Saïdi was recipient of an EMPRO fellowship. %< avec comité de lecture %@ 1742-6405 %J AIDS Research and Therapy %I BioMed Central %V 7 %N 1 %P 16 %8 2010 %D 2010 %R 10.1186/1742-6405-7-16 %M 20546571 %Z Life Sciences [q-bio]/Human health and pathology/Infectious diseasesJournal articles %X BACKGROUND: HIV-1 in genital secretions may be opsonized by several molecules including complement components. Opsonized HIV-1 by complement enhances the infection of various mucosal target cells, such as dendritic cells (DC) and epithelial cells. RESULTS: We herein evaluated the effect of HIV-1 complement opsonization on microbicide candidates' activity, by using three in vitro mucosal models: CCR5-tropic HIV-1JR-CSF transcytosis through epithelial cells, HIV-1JR-CSF attachment on immature monocyte-derived dendritic cells (iMDDC), and infectivity of iMDDC by CCR5-tropic HIV-1BaL and CXCR4-tropic HIV-1NDK. A panel of 10 microbicide candidates [T20, CADA, lectines HHA & GNA, PVAS, human lactoferrin, and monoclonal antibodies IgG1B12, 12G5, 2G12 and 2F5], were investigated using cell-free unopsonized or opsonized HIV-1 by complements. Only HHA and PVAS were able to inhibit HIV trancytosis. Upon opsonization, transcytosis was affected only by HHA, HIV-1 adsorption on iMDDC by four molecules (lactoferrin, IgG1B12, IgG2G5, IgG2G12), and replication in iMDDC of HIV-1BaL by five molecules (lactoferrin, CADA, T20, IgG1B12, IgG2F5) and of HIV-1NDK by two molecules (lactoferrin, IgG12G5). CONCLUSION: These observations demonstrate that HIV-1 opsonization by complements may modulate in vitro the efficiency of candidate microbicides to inhibit HIV-1 infection of mucosal target cells, as well as its crossing through mucosa. %G English %2 https://inserm.hal.science/inserm-00663893/document %2 https://inserm.hal.science/inserm-00663893/file/1742-6405-7-16.pdf %L inserm-00663893 %U https://inserm.hal.science/inserm-00663893 %~ INSERM %~ UNIV-PARIS5 %~ CNRS %~ UNIV-PICARDIE %~ APHP %~ CHU-UNIV-PARIS5 %~ UNIV-PARIS %~ U-PICARDIE