%0 Journal Article %T Nucleosome rotational setting is associated with transcriptional regulation in promoters of tissue-specific human genes. %+ Institut de biologie de l'ENS Paris (IBENS) %A Hebert, Charles %A Roest Crollius, Hugues %Z This work is funded by the ATIP program of the Centre National de la Recherche Scientifique (HRC) and by the French Ministère de l'Enseignement Supérieur et de la Recherche (CH). %< avec comité de lecture %@ 1465-6906 %J Genome Biology %I BioMed Central %V 11 %N 5 %P R51 %8 2010 %D 2010 %R 10.1186/gb-2010-11-5-r51 %M 20462404 %Z Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN] %Z Life Sciences [q-bio]/GeneticsJournal articles %X BACKGROUND: The position of a nucleosome, both translational along the DNA molecule and rotational between the histone core and the DNA, is controlled by many factors, including the regular occurrence of specific dinucleotides with a period of approximately 10 bp, important for the rotational setting of the DNA around the histone octamer. RESULTS: We show that such a 10 bp periodic signal of purine-purine dinucleotides occurs in phase with the transcription start site (TSS) of human genes and is centered on the position of the first (+1) nucleosome downstream of the TSS. These data support a direct link between transcription and the rotational setting of the nucleosome. The periodic signal is most prevalent in genes that contain CpG islands that are expressed at low levels in a tissue-specific manner and are involved in the control of transcription. CONCLUSIONS: These results, together with several lines of evidence from the recent literature, support a new model whereby the +1 nucleosome could be more efficiently disassembled from gene promoters by H3K56 acetylation marks if the periodic signal specifies an optimal rotational setting. %G English %2 https://inserm.hal.science/inserm-00663747/document %2 https://inserm.hal.science/inserm-00663747/file/gb-2010-11-5-r51.pdf %L inserm-00663747 %U https://inserm.hal.science/inserm-00663747 %~ INSERM %~ ENS-PARIS %~ CNRS %~ PSL %~ ENS-PSL