%0 Journal Article %T Adaptation to statins restricts human tumour growth in Nude mice. %+ Génétique moléculaire et génétique épidémiologique %+ Tumeurs endocrines digestives : mécanismes de la tumorigenèse et de la progression tumorale %+ C.RIS Pharma %A Follet, Julie %A Rémy, Lionel %A Hesry, Vincent %A Simon, Brigitte %A Gillet, Danièle %A Auvray, Pierrick %A Corcos, Laurent %A Le Jossic-Corcos, Catherine %Z This work was supported by the INSERM, the IFR 148 ScInBioS, the Cancéropôle Grand Ouest, the Ligue régionale contre le Cancer, the FEDER (Fonds Européen de Développement Régional) funds [grant number PRESAGE 9511]; the Brittany Region [project number XCOR 5012838]; the CRITT (Centre Régional d'Innovation et de Transfert de Technologie dans le domaine de la Santé) Santé Bretagne [project number CASAC 2935]; the University of Brest (institutional university grant); the Medical Faculty of the University Hospital of Brest (Interface contract to Laurent Corcos) and the ANIPATH laboratory of the Faculty of Medicine Laënnec (Lyon). Julie Follet was supported by a fellowship from the Brittany region (Allocation de Recherche Doctorale). %< avec comité de lecture %@ 1471-2407 %J BMC Cancer %I BioMed Central %V 11 %N 1 %P 491 %8 2011-11-22 %D 2011 %R 10.1186/1471-2407-11-491 %M 22107808 %K Statins %K Gastric cancer %K Nude mice %K Apoptosis %K Angiogenesis %Z Life Sciences [q-bio]/CancerJournal articles %X ABSTRACT: BACKGROUND: Statins have long been used as anti-hypercholesterolemia drugs, but numerous lines of evidence suggest that they may also bear anti-tumour potential. We have recently demonstrated that it was possible to isolate cancer cells adapted to growth in the continuous presence of lovastatin. These cells grew more slowly than the statin-sensitive cells of origin. In the present study, we compared the ability of both statin-sensitive and statin-resistant cells to give rise to tumours in Nude mice. METHODS: HGT-1 human gastric cancer cells and L50 statin-resistant derivatives were injected subcutaneously into Nude mice and tumour growth was recorded. At the end of the experiment, tumours were recovered and marker proteins were analyzed by western blotting, RT-PCR and immunohistochemistry. RESULTS: L50 tumours grew more slowly, showed a strong decrease in cyclin B1, over-expressed collagen IV, and had reduced laminin 332, VEGF and CD34 levels, which, collectively, may have restricted cell division, cell adhesion and neoangiogenesis. CONCLUSIONS: Taken together, these results showed that statin-resistant cells developed into smaller tumours than statin-sensitive cells. This may be reflective of the cancer restricting activity of statins in humans, as suggested from several retrospective studies with subjects undergoing statin therapy for several years. %G English %2 https://inserm.hal.science/inserm-00658366/document %2 https://inserm.hal.science/inserm-00658366/file/1471-2407-11-491.pdf %L inserm-00658366 %U https://inserm.hal.science/inserm-00658366 %~ INSERM %~ UNIV-BREST %~ UNIV-LYON1 %~ UDL %~ UNIV-LYON