Differentiation associated regulation of microRNA expression in vivo in human CD8+ T cell subsets. - Inserm - Institut national de la santé et de la recherche médicale Access content directly
Journal Articles Journal of Translational Medicine Year : 2011

Differentiation associated regulation of microRNA expression in vivo in human CD8+ T cell subsets.

Bruno Salaun
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  • PersonId : 915466
Takuya Yamamoto
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  • PersonId : 915467
Yasuko Tsunetsugu-Yokota
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  • PersonId : 915469
Antoine Roux
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  • PersonId : 915470
Lukas Baitsch
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  • PersonId : 915471
Redouane Rouas
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  • PersonId : 915472
Hussein Fayyad-Kazan
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  • PersonId : 915473
Petra Baumgaertner
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  • PersonId : 915474
Anirudh Ramesh
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  • PersonId : 915475
Marion Braun
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  • PersonId : 915476
Daniel Speiser
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  • PersonId : 915477
Philippe Martiat
  • Function : Author
  • PersonId : 915478
Victor Appay
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  • PersonId : 871208


BACKGROUND: The differentiation of CD8+ T lymphocytes following priming of naïve cells is central in the establishment of the adaptive immune response. Yet, the molecular events underlying this process are not fully understood. MicroRNAs have been recently shown to play a key role in the regulation of haematopoiesis in mouse, but their implication in peripheral lymphocyte differentiation in humans remains largely unknown. METHODS: In order to explore the potential implication of microRNAs in CD8+ T cell differentiation in humans, microRNA expression profiles were analysed using microarrays and quantitative PCR in several human CD8+ T cell subsets defining the major steps of the T cell differentiation pathway. RESULTS: We found expression of a limited set of microRNAs, including the miR-17~92 cluster. Moreover, we reveal the existence of differentiation-associated regulation of specific microRNAs. When compared to naive cells, miR-21 and miR-155 were indeed found upregulated upon differentiation to effector cells, while expression of the miR-17~92 cluster tended to concomitantly decrease. CONCLUSIONS: This study establishes for the first time in a large panel of individuals the existence of differentiation associated regulation of microRNA expression in human CD8+ T lymphocytes in vivo, which is likely to impact on specific cellular functions.
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inserm-00649413 , version 1 (07-12-2011)



Bruno Salaun, Takuya Yamamoto, Bassam Badran, Yasuko Tsunetsugu-Yokota, Antoine Roux, et al.. Differentiation associated regulation of microRNA expression in vivo in human CD8+ T cell subsets.. Journal of Translational Medicine, 2011, 9 (1), pp.44. ⟨10.1186/1479-5876-9-44⟩. ⟨inserm-00649413⟩
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