%0 Journal Article %T CD4 ligation excludes the Carma1-Bcl10-MALT1 complex from GM1-positive membrane rafts in CD3/CD28 activated T cells. %+ Institut de recherche en cancérologie de Montpellier (IRCM - U896 Inserm - UM1) %A Rigo, Maxime %A Chentouf, Myriam %A Pèlegrin, André %A Chardès, Thierry %Z Ligue Nationale contre le Cancer, Comité de l'Hérault, and the Association de Recherche contre le Cancer. %< avec comité de lecture %@ 0008-8749 %J Cellular Immunology %I Elsevier %V 270 %N 1 %P 40-6 %8 2011 %D 2011 %R 10.1016/j.cellimm.2011.03.025 %M 21489413 %K antibody %K raft %K signalling %K CD4 %K Carma1 %K cancer %Z Life Sciences [q-bio]/Cancer %Z Life Sciences [q-bio]/Immunology %Z Life Sciences [q-bio]/Biotechnology %Z Computer Science [cs]/Biotechnology %Z Life Sciences [q-bio]/Cellular BiologyJournal articles %X The antibody 13B8.2, which is directed against the CDR3-like loop on the D1 domain of CD4, induces CD4/ZAP-70 reorganization and ceramide release in membrane rafts. Here, we investigated whether CD4/ZAP-70 compartmentalization could be mediated by an effect of 13B8.2 on the Carma1-Bcl10-MALT1 complex in membrane rafts. We report that treatment of CD3/CD28-activated Jurkat T cells with 13B8.2, but not rituximab, excluded Carma1-Bcl10-MALT1 proteins from GM1(+) membrane rafts and concomitantly decreased NF-κB activation. Fluorescence confocal imaging confirmed that Carma1-Bcl10 and Carma1-MALT1 co-patching, observed in GM1(+) membrane rafts following CD3/CD28 activation, were abrogated after a 24h-treatment with 13B8.2. The CD4/ZAP-70 compartmentalization in membrane rafts induced by 13B8.2 is thus related to Carma1-Bcl10-MALT1 raft exclusion. %G English %2 https://inserm.hal.science/inserm-00588709/document %2 https://inserm.hal.science/inserm-00588709/file/Revised_Ms_CIMM-10-182_Rigo_et_al.pdf %L inserm-00588709 %U https://inserm.hal.science/inserm-00588709 %~ INSERM %~ UNIV-MONTP1 %~ FNCLCC %~ VALDAURELLE %~ BS %~ UNIV-MONTPELLIER %~ IRCM %~ UM1-UM2 %~ UM-2015-2021