%0 Journal Article %T Calcium-dependent regulation of SNARE-mediated membrane fusion by calmodulin. %+ Unité de Neurobiologie des canaux Ioniques et de la Synapse (UNIS - Inserm U1072) %+ Centre de recherche en neurobiologie - neurophysiologie de Marseille (CRN2M) %A Di Giovanni, Jerome %A Iborra, Cécile %A Maulet, Yves %A Lévêque, Christian %A El Far, Oussama %A Seagar, Michael %< avec comité de lecture %@ 0021-9258 %J Journal of Biological Chemistry %I American Society for Biochemistry and Molecular Biology %V 285 %N 31 %P 23665-75 %8 2010-07-30 %D 2010 %R 10.1074/jbc.M109.096073 %M 20519509 %Z Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]Journal articles %X Neuroexocytosis requires SNARE proteins, which assemble into trans complexes at the synaptic vesicle/plasma membrane interface and mediate bilayer fusion. Ca(2+) sensitivity is thought to be conferred by synaptotagmin, although the ubiquitous Ca(2+)-effector calmodulin has also been implicated in SNARE-dependent membrane fusion. To examine the molecular mechanisms involved, we examined the direct action of calmodulin and synaptotagmin in vitro, using fluorescence resonance energy transfer to assay lipid mixing between target- and vesicle-SNARE liposomes. Ca(2+)/calmodulin inhibited SNARE assembly and membrane fusion by binding to two distinct motifs located in the membrane-proximal regions of VAMP2 (K(D) = 500 nm) and syntaxin 1 (K(D) = 2 microm). In contrast, fusion was increased by full-length synaptotagmin 1 anchored in vesicle-SNARE liposomes. When synaptotagmin and calmodulin were combined, synaptotagmin overcame the inhibitory effects of calmodulin. Furthermore, synaptotagmin displaced calmodulin binding to target-SNAREs. These findings suggest that two distinct Ca(2+) sensors act antagonistically in SNARE-mediated fusion. %G English %L inserm-00581206 %U https://inserm.hal.science/inserm-00581206 %~ INSERM %~ CNRS %~ UNIV-AMU