%0 Journal Article %T Direct genotyping of cytomegalovirus envelope glycoproteins from toddler's saliva samples %+ Biologie moléculaire et cellulaire des microorganismes (EA3175) %+ Service de Bactériologie, Virologie, Hygiène [CHU Limoges] %+ Centre de Physiopathologie Toulouse Purpan (CPTP) %+ Service de Pédiatrie médicale [CHU Limoges] %+ Association Française de Pédiatrie Ambulatoire (AFPA) %A Grosjean, Jérôme %A Hantz, Sébastien %A Cotin, Sébastien %A Baclet, Marie-Claire %A Mengelle, Catherine %A Trapes, L. %A Virey, B. %A Undreiner, F. %A Brosset, P. %A Pasquier, C. %A Denis, François %A Alain, Sophie %Z Centre Hospitalier Universitaire (CHU) Limoges, Centre National de Reference du CMV, Laboratoire de Bactériologie-Virologie-Hygiène, Limoges, France %< avec comité de lecture %J J Clin Virol %V 46 Suppl 4 %P S43-8 %8 2009-12 %D 2009 %R 10.1016/j.jcv.2009.08.018 %M 19805004 %Z Life Sciences [q-bio]/Human health and pathology/Infectious diseases %Z Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology %Z Life Sciences [q-bio]/Microbiology and Parasitology/VirologyJournal articles %X BACKGROUND: The polymorphism of genes encoding CMV envelope protein is used for strain classification and may influence pathogenesis and/or infectivity. CMV genotyping is usually based on sequencing or acrylamide gel-RFLP, but these methods are not suited to rapid screening of large populations. OBJECTIVES: We developed a high-throughput method to analyze CMV strains diversity and to detect multiple-strain infection in a large population of toddlers (six daycare centers (DCC) and an emergency unit (EU)). METHODS: We developed a new PCR-RFLP method coupled with capillary electrophoresis fragment detection for UL55-gB, UL75-gH and UL73-gN genotyping. To detect gB recombinants, gpUL55 typing was applied to two variable zones (NTerminal and central). We applied this method to 212 CMV-positive saliva samples and controlled the results by direct sequencing of PCR products. RESULTS: We identified 112 strains, that fell into eight groups in UL55-gB, two groups in UL75-gH, and seven groups in UL73-gN. The 79 samples from the emergency unit contained 30 strains, 28 children harboring 2 strains. The samples (n=133) from the six daycare centers contained respectively 4, 1, 6, 1 and 11 strains. Fifteen percent of strains were UL55-gB recombinants. CONCLUSION: Our new method can simultaneously determine gB, gH and gN genotypes and offers more precise classification of CMV strains than previous RFLP-based methods. This could constitute the basis for a new classification, particularly in UL55-gB. Easy direct identification of multiple strains and recombinants in pathological samples could facilitate large epidemiologic studies. %G English %L inserm-00533091 %U https://inserm.hal.science/inserm-00533091 %~ INSERM %~ UNILIM %~ UNIV-TLSE3 %~ CNRS %~ UMR-S1092 %~ GEIST %~ INFINITY %~ OMEGAHEALTH %~ UNIV-UT3 %~ UT3-INP %~ UT3-TOULOUSEINP