%0 Journal Article %T Using lentiviral vectors for efficient pancreatic cancer gene therapy. %+ Métabolisme Plasticité et Mitochondrie [lié à l'ex IFR 31] (LMPM) %+ Institut de médecine moléculaire de Rangueil (I2MR) %+ CIC - Biotherapie - Toulouse %+ Pôle Maladies de l'appareil digestif [CHU Toulouse] %A Ravet, Emmanuel %A Lulka, Hubert %A Gross, F. %A Casteilla, Louis %A Buscail, Louis %A Cordelier, Pierre %< avec comité de lecture %@ 0929-1903 %J Cancer Gene Therapy %I Nature Publishing Group %V 17 %N 5 %P 315-24 %8 2010-05 %D 2010 %R 10.1038/cgt.2009.79 %M 19911032 %Z Life Sciences [q-bio]/EthicsJournal articles %X Pancreatic cancer (PC) remains a life-threatening disease. Efficient therapeutic gene delivery to PC-derived cells continues to present challenges. We used self-inactivated lentiviral vectors to transduce PC-derived cells in vitro and in vivo. We showed that lentiviral vectors transduce PC-derived cell lines with high efficiency (>90%), regardless of the differentiation state of the cell. Next, we transferred human interferon beta (hIFN-beta) gene. Expression of hIFN-beta in PC cells using lentiviral vectors resulted in the inhibition of cell proliferation and the induction of cell death by apoptosis. In vivo, lentiviral administration of hIFN-beta prevented PC tumor progression for up to 15 days following gene therapy, and induced tumor regression/stabilization in 50% of the mice treated. Again, hIFN-beta expression resulted in cancer cell proliferation inhibition and apoptosis induction. We provide evidence that human immunodeficiency virus (HIV)-1-based lentiviral vectors are very efficient for gene transfer in PC-derived cells in vitro and in vivo. As a consequence, delivery of hIFN-beta stopped PC tumor progression. Thus, our approach could be applied to the 85% of PC patients with a locally advanced disease. %G English %L inserm-00480870 %U https://inserm.hal.science/inserm-00480870 %~ INSERM %~ UNIV-TLSE3 %~ CNRS %~ ETHIQUE %~ UNIV-UT3 %~ UT3-INP %~ UT3-TOULOUSEINP %~ TEST3-HALCNRS