Mineralocorticoid modulation of cardiac ryanodine receptor activity is associated with downregulation of FK506-binding proteins. - Inserm - Institut national de la santé et de la recherche médicale Access content directly
Journal Articles Circulation Year : 2009

Mineralocorticoid modulation of cardiac ryanodine receptor activity is associated with downregulation of FK506-binding proteins.

Ana María Gómez
Roxane Schaub
Emeline Perrier
Sylvain Richard
Frederic Jaisser

Abstract

BACKGROUND: The mineralocorticoid pathway is involved in cardiac arrhythmias associated with heart failure through mechanisms that are incompletely understood. Defective regulation of the cardiac ryanodine receptor (RyR) is an important cause of the initiation of arrhythmias. Here, we examined whether the aldosterone pathway might modulate RyR function. METHODS AND RESULTS: Using the whole-cell patch clamp method, we observed an increase in the occurrence of delayed afterdepolarizations during action potential recordings in isolated adult rat ventricular myocytes exposed for 48 hours to aldosterone 100 nmol/L, in freshly isolated myocytes from transgenic mice with human mineralocorticoid receptor expression in the heart, and in wild-type littermates treated with aldosterone. Sarcoplasmic reticulum Ca(2+) load and RyR expression were not altered; however, RyR activity, visualized in situ by confocal microscopy, was increased in all cells, as evidenced by an increased occurrence and redistribution to long-lasting and broader populations of spontaneous Ca(2+) sparks. These changes were associated with downregulation of FK506-binding proteins (FKBP12 and 12.6), regulatory proteins of the RyR macromolecular complex. CONCLUSIONS: We suggest that in addition to modulation of Ca(2+) influx, overstimulation of the cardiac mineralocorticoid pathway in the heart might be a major upstream factor for aberrant Ca(2+) release during diastole, which contributes to cardiac arrhythmia in heart failure.
Fichier principal
Vignette du fichier
AMGomezCIRCULATIONAHA-2008-805804finalrevison.pdf (166.59 Ko) Télécharger le fichier
Origin : Files produced by the author(s)
Loading...

Dates and versions

inserm-00422024 , version 1 (05-10-2009)

Identifiers

Cite

Ana María Gómez, Angélica Rueda, Yannis Sainte-Marie, Laetitia Pereira, Spyros Zissimopoulos, et al.. Mineralocorticoid modulation of cardiac ryanodine receptor activity is associated with downregulation of FK506-binding proteins.. Circulation, 2009, 119 (16), pp.2179-87. ⟨10.1161/CIRCULATIONAHA.108.805804⟩. ⟨inserm-00422024⟩
837 View
1217 Download

Altmetric

Share

Gmail Facebook X LinkedIn More